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Journal of Clinical Microbiology, May 2001, p. 1771-1773, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1771-1773.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Hepatitis C Virus Genotyping Based on 5' Noncoding Sequence Analysis (Trugene)

P. Halfon,1,* P. Trimoulet,2 M. Bourliere,3 H. Khiri,1 V. de Lédinghen,2 P. Couzigou,2 J. M. Feryn,1 P. Alcaraz,1 C. Renou,4 H. J. A. Fleury,2 and D. Ouzan5

Département de Virologie, Laboratoire Alphabio,1 and Département d'Hépato-Gastroentérologie, Hôpital Saint Joseph,3 Marseille, Département de Virologie et d'Hépatologie, CHU Bordeaux,2 Département d'Hépato-Gastroentérologie, CH Hyéres,4 and Institut Arnault Tzanck, Saint Laurent du Var,5 France

Received 26 July 2000/Returned for modification 26 September 2000/Accepted 5 March 2001

Hepatitis C virus (HCV) genotyping of samples from 184 patients with chronic HCV infection by the Trugene 5'NC genotyping kit, based on sequence analysis of the 5' noncoding region (5' NCR), and the InnoLiPA assay was evaluated. In addition to these methods, the 184 samples were also analyzed by sequencing of part of the NS5B of the HCV genome after in-house PCR amplification, as a means of validating results obtained with the 5' NCR. The distribution of the genotypes typed by NS5B sequence analysis was as follows: 1a, 41 samples; 1b, 58 samples; 1d, 1 sample; 2a, 5 samples; 2b, 2 samples; 2c, 7 samples; 3a, 46 samples; 4a, 7 samples; 4c, 1 samples; 4e, 9 samples; 5a, 6 samples; 6a, 1 sample. The Trugene and InnoLiPA assays gave concordant results within HCV types in 100% of cases. The ability to discriminate at the subtype level was 76 and 74% for the Trugene and the InnoLiPA assays, respectively.

* Corresponding author. Mailing address: Laboratoire Alphabio, 23 Rue de Friedland, Hôpital Ambroise Paré, 13006 Marseille, France. Phone: (33) 4 91 25 41 00. Fax: (33) 4 91 79 20 44. E-mail: philippe.halfon{at}alphabio.fr.

Journal of Clinical Microbiology, May 2001, p. 1771-1773, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1771-1773.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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