JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Halfon, P.
Right arrow Articles by Ouzan, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Halfon, P.
Right arrow Articles by Ouzan, D.

Journal of Clinical Microbiology, May 2001, p. 1771-1773, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1771-1773.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Hepatitis C Virus Genotyping Based on 5' Noncoding Sequence Analysis (Trugene)

P. Halfon,1,* P. Trimoulet,2 M. Bourliere,3 H. Khiri,1 V. de Lédinghen,2 P. Couzigou,2 J. M. Feryn,1 P. Alcaraz,1 C. Renou,4 H. J. A. Fleury,2 and D. Ouzan5

Département de Virologie, Laboratoire Alphabio,1 and Département d'Hépato-Gastroentérologie, Hôpital Saint Joseph,3 Marseille, Département de Virologie et d'Hépatologie, CHU Bordeaux,2 Département d'Hépato-Gastroentérologie, CH Hyéres,4 and Institut Arnault Tzanck, Saint Laurent du Var,5 France

Received 26 July 2000/Returned for modification 26 September 2000/Accepted 5 March 2001

Hepatitis C virus (HCV) genotyping of samples from 184 patients with chronic HCV infection by the Trugene 5'NC genotyping kit, based on sequence analysis of the 5' noncoding region (5' NCR), and the InnoLiPA assay was evaluated. In addition to these methods, the 184 samples were also analyzed by sequencing of part of the NS5B of the HCV genome after in-house PCR amplification, as a means of validating results obtained with the 5' NCR. The distribution of the genotypes typed by NS5B sequence analysis was as follows: 1a, 41 samples; 1b, 58 samples; 1d, 1 sample; 2a, 5 samples; 2b, 2 samples; 2c, 7 samples; 3a, 46 samples; 4a, 7 samples; 4c, 1 samples; 4e, 9 samples; 5a, 6 samples; 6a, 1 sample. The Trugene and InnoLiPA assays gave concordant results within HCV types in 100% of cases. The ability to discriminate at the subtype level was 76 and 74% for the Trugene and the InnoLiPA assays, respectively.

* Corresponding author. Mailing address: Laboratoire Alphabio, 23 Rue de Friedland, Hôpital Ambroise Paré, 13006 Marseille, France. Phone: (33) 4 91 25 41 00. Fax: (33) 4 91 79 20 44. E-mail: philippe.halfon{at}alphabio.fr.

Journal of Clinical Microbiology, May 2001, p. 1771-1773, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1771-1773.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.