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Journal of Clinical Microbiology, May 2001, p. 1791-1795, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1791-1795.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Results Generated by Serotyping, Pulsed-Field Restriction Analysis, Ribotyping, and Repetitive-Sequence PCR Used To Characterize Penicillin-Resistant Pneumococci from the United States

W. Michael Dunne Jr.,1,* Karen Sue Kehl,2 Carol A. Holland-Staley,3 Angela B. Brueggemann,4 Michael A. Pfaller,4 and Gary V. Doern4

Department of Pathology and Immunology, Washington University School of Medicine, St. Louis, Missouri1; Department of Pathology, Medical College of Wisconsin, Milwaukee, Wisconsin2; Division of Infectious Diseases, Henry Ford Hospital, Detroit, Michigan3; and Department of Pathology, University of Iowa College of Medicine, Iowa City, Iowa4

Received 8 September 2000/Returned for modification 20 October 2000/Accepted 24 February 2001

One hundred forty-seven isolates of Streptococcus pneumoniae with high-level penicillin resistance collected during a national surveillance program in the United States were characterized by serotyping, pulsed-field restriction analysis, ribotyping, and repetitive-sequence (BOX element) PCR. The results generated by each method were compared by frequency of association to examine whether relationships existed between the various typing methods and statistically to determine association with the geographic source of the isolate or the age of the patient from whom the isolate was obtained. When the data were examined by pairwise analysis of individual strain classifications produced by each typing method, no statistically significant relationships between strain type, geographic location, or patient age were identified, suggesting that distinct clones of penicillin-resistant S. pneumoniae have been widely distributed throughout the United States. However, we did observed shared expression of two or three typing markers at a high frequency (>50%) among clusters of strains, indicating a certain level of concordance between the various typing methods used to classify penicillin-resistant S. pneumoniae.

* Corresponding author. Mailing address: Division of Laboratory Medicine, Department of Pathology and Immunology, Washington University School of Medicine, 660 South Euclid Ave., Box 8118, St. Louis, MO 63110. Phone: (314) 362-2998. Fax: (314) 362-1461. E-mail: dunne{at}labmed.wustl.edu.

Journal of Clinical Microbiology, May 2001, p. 1791-1795, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1791-1795.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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