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Journal of Clinical Microbiology, May 2001, p. 1808-1812, Vol. 39, No. 5
Projet RETRO-CI, CHU de Treichville, Abidjan,
Ivory Coast,1 and Division of HIV/AIDS
Prevention, National Center for HIV, STD, and TB Prevention,
Centers for Disease Control and Prevention, Atlanta,
Georgia2
Received 13 November 2000/Returned for modification 29 January
2001/Accepted 28 February 2001
To evaluate serologic testing algorithms for human immunodeficiency
virus (HIV) based on a combination of rapid assays among persons with
HIV-1 (non-B subtypes) infection, HIV-2 infection, and HIV-1-HIV-2
dual infections in Abidjan, Ivory Coast, a total of 1,216 sera with
known HIV serologic status were used to evaluate the sensitivity and
specificity of four rapid assays: Determine HIV-1/2, Capillus
HIV-1/HIV-2, HIV-SPOT, and Genie II HIV-1/HIV-2. Two serum panels
obtained from patients recently infected with HIV-1 subtypes B and
non-B were also included. Based on sensitivity and specificity, three
of the four rapid assays were evaluated prospectively in parallel
(serum samples tested by two simultaneous rapid assays) and serial
(serum samples tested by two consecutive rapid assays) testing
algorithms. All assays were 100% sensitive, and specificities ranged
from 99.4 to 100%. In the prospective evaluation, both the parallel
and serial algorithms were 100% sensitive and specific.
Our results suggest that rapid assays have high sensitivity and
specificity and, when used in parallel or serial testing
algorithms, yield results similar to those of enzyme-linked
immunosorbent assay-based testing strategies. HIV serodiagnosis
based on rapid assays may be a valuable alternative in implementing HIV
prevention and surveillance programs in areas where sophisticated
laboratories are difficult to establish.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.1808-1812.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Sensitivity and Specificity of Human Immunodeficiency Virus Rapid
Serologic Assays and Testing Algorithms in an Antenatal Clinic in
Abidjan, Ivory Coast
*
Corresponding author: Mailing address: Laboratory of
Virology, Projet RETRO-CI, 01 BP 1712, Abidjan 01, Ivory Coast. Phone: 225-21 25 41 89. Fax: 225-21 24 29 69. E-mail:
jcn5{at}cdc.gov.
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