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Journal of Clinical Microbiology, May 2001, p. 1840-1844, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1840-1844.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Simple Method for Determining Biovar and Serovar Types of Ureaplasma urealyticum Clinical Isolates Using PCR-Single-Strand Conformation Polymorphism Analysis

David Pitcher,1,* Margaret Sillis,2 and Janet A. Robertson3

Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, London NW9 5HT,1 and Public Health Laboratory, Norwich NR2 3TX,2 United Kingdom, and Department of Medical Microbiology and Immunology, University of Alberta, Edmonton, Alberta T6G 2H7, Canada3

Received 29 September 2000/Returned for modification 9 January 2001/Accepted 23 February 2001

Ureaplasma urealyticum has been associated with urethritis in men, obstetric problems in women, and respiratory distress syndrome in preterm infants. U. urealyticum can be divided into two biovars comprising 14 serovars. Partial sequences of genes encoding the multiple-banded antigens of the cell surface are known. Using a commercially available precast DNA mutation detection gel system, we have developed a simple and reproducible PCR-single-strand conformation polymorphism analysis method for differentiating the biovars of this species that reveals five patterns among the 14 serovars and enables clinical isolates to be typed directly from broth cultures.

* Corresponding author. Mailing address: Respiratory and Systemic Infection Laboratory, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 (0) 208 200 4400. Fax: 44 (0) 208 205 6528. E-mail: dpitcher{at}phls.nhs.uk.

Journal of Clinical Microbiology, May 2001, p. 1840-1844, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1840-1844.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Waites, K. B., Katz, B., Schelonka, R. L. (2005). Mycoplasmas and Ureaplasmas as Neonatal Pathogens. Clin. Microbiol. Rev. 18: 757-789 [Abstract] [Full Text]  


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