This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holguín, A.
Right arrow Articles by Soriano, V.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holguín, A.
Right arrow Articles by Soriano, V.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2001, p. 1850-1854, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1850-1854.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Prevalence of Human Immunodeficiency Virus Type 1 (HIV-1) Non-B Subtypes in Foreigners Living in Madrid, Spain, and Comparison of the Performances of the AMPLICOR HIV-1 MONITOR Version 1.0 and the New Automated Version 1.5

Africa Holguín,1 Belén Aracil,2 Amparo Álvarez,1 Carlos Barros,2 and Vincent Soriano1,*

Service of Infectious Diseases, Hospital Carlos III, Instituto de Salud Carlos III,1 and Service of Microbiology and HIV Unit, Hospital de Móstoles,2 Madrid, Spain

Received 10 July 2000/Returned for modification 16 October 2000/Accepted 21 February 2001

Plasma specimens collected in 1999 from 32 human immunodeficiency virus type 1 (HIV-1)-infected foreigners living in Madrid, Spain, were examined for the presence of non-B subtypes. Furthermore, plasma viremia was quantified using two different AMPLICOR HIV-1 MONITOR tests, version 1.0 and the new upgraded and automated version 1.5 (COBAS). Most patients came from Africa, where they most likely had acquired HIV-1 infection through sexual contact. HIV-1 genetic subtyping was based on the phylogenetic analysis of the protease gene. Twenty-two subtype B, six subtype G, two subtype C, one subtype A, and one D subtype infection were found. Overall, non-B subtypes represented 31.25% of the study population. Irrespective of the HIV-1 variant, viral load values above the detection limit (200 HIV RNA copies/ml) increased from 56.2 to 71.9% for results obtained using MONITOR version 1.0 and COBAS, respectively. Moreover, significant differences in viral load values (>0.5 logs) were recognized in up to 37.5% of samples. In summary, COBAS seemed to be more reliable for testing plasma viral load in HIV-infected immigrants living in Spain, one third of whom carried non-B subtypes.

* Corresponding author. Mailing address: C/ Nueva Zelanda 54, 4° B, 28035 Madrid, Spain. Phone: 34-91 4532500. Fax: 34-91 733 6614. E-mail: vsoriano{at}dragonet.es.

Journal of Clinical Microbiology, May 2001, p. 1850-1854, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1850-1854.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Holguin, A., Lopez, M., Molinero, M., Soriano, V. (2008). Performance of Three Commercial Viral Load Assays, Versant Human Immunodeficiency Virus Type 1 (HIV-1) RNA bDNA v3.0, Cobas AmpliPrep/Cobas TaqMan HIV-1, and NucliSens HIV-1 EasyQ v1.2, Testing HIV-1 Non-B Subtypes and Recombinant Variants. J. Clin. Microbiol. 46: 2918-2923 [Abstract] [Full Text]  
  • Foulongne, V., Montes, B., Didelot-Rousseau, M.-N., Segondy, M. (2006). Comparison of the LCx Human Immunodeficiency Virus (HIV) RNA Quantitative, RealTime HIV, and COBAS AmpliPrep-COBAS TaqMan Assays for Quantitation of HIV Type 1 RNA in Plasma.. J. Clin. Microbiol. 44: 2963-2966 [Abstract] [Full Text]  
  • Tobin, N. H., Learn, G. H., Holte, S. E., Wang, Y., Melvin, A. J., McKernan, J. L., Pawluk, D. M., Mohan, K. M., Lewis, P. F., Mullins, J. I., Frenkel, L. M. (2005). Evidence that Low-Level Viremias during Effective Highly Active Antiretroviral Therapy Result from Two Processes: Expression of Archival Virus and Replication of Virus. J. Virol. 79: 9625-9634 [Abstract] [Full Text]  
  • Brambilla, D. J., Jennings, C., Morack, R., Granger, S., Bremer, J. W. (2004). Comparison of the Sensitivities of the Version 1.5 and Version 1.0 Ultrasensitive Roche AMPLICOR HIV-1 MONITOR Kits at Low Concentrations of Human Immunodeficiency Virus RNA. J. Clin. Microbiol. 42: 2819-2820 [Abstract] [Full Text]  
  • Holzl, G., Stocher, M., Leb, V., Stekel, H., Berg, J. (2003). Entirely Automated Quantification of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Plasma by Using the Ultrasensitive COBAS AMPLICOR HIV-1 Monitor Test and RNA Purification on the MagNA Pure LC Instrument. J. Clin. Microbiol. 41: 1248-1251 [Abstract] [Full Text]  
  • Martin, J C, Holguin, A, Soriano, V (2002). Prevalence of different HIV-1 subtypes in an urban clinic in Madrid. Sex. Transm. Infect. 78 : e1-e1 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»