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Journal of Clinical Microbiology, May 2001, p. 1850-1854, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1850-1854.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Prevalence of Human Immunodeficiency Virus Type 1 (HIV-1) Non-B Subtypes in Foreigners Living in Madrid, Spain, and Comparison of the Performances of the AMPLICOR HIV-1 MONITOR Version 1.0 and the New Automated Version 1.5

Africa Holguín,1 Belén Aracil,2 Amparo Álvarez,1 Carlos Barros,2 and Vincent Soriano1,*

Service of Infectious Diseases, Hospital Carlos III, Instituto de Salud Carlos III,1 and Service of Microbiology and HIV Unit, Hospital de Móstoles,2 Madrid, Spain

Received 10 July 2000/Returned for modification 16 October 2000/Accepted 21 February 2001

Plasma specimens collected in 1999 from 32 human immunodeficiency virus type 1 (HIV-1)-infected foreigners living in Madrid, Spain, were examined for the presence of non-B subtypes. Furthermore, plasma viremia was quantified using two different AMPLICOR HIV-1 MONITOR tests, version 1.0 and the new upgraded and automated version 1.5 (COBAS). Most patients came from Africa, where they most likely had acquired HIV-1 infection through sexual contact. HIV-1 genetic subtyping was based on the phylogenetic analysis of the protease gene. Twenty-two subtype B, six subtype G, two subtype C, one subtype A, and one D subtype infection were found. Overall, non-B subtypes represented 31.25% of the study population. Irrespective of the HIV-1 variant, viral load values above the detection limit (200 HIV RNA copies/ml) increased from 56.2 to 71.9% for results obtained using MONITOR version 1.0 and COBAS, respectively. Moreover, significant differences in viral load values (>0.5 logs) were recognized in up to 37.5% of samples. In summary, COBAS seemed to be more reliable for testing plasma viral load in HIV-infected immigrants living in Spain, one third of whom carried non-B subtypes.

* Corresponding author. Mailing address: C/ Nueva Zelanda 54, 4° B, 28035 Madrid, Spain. Phone: 34-91 4532500. Fax: 34-91 733 6614. E-mail: vsoriano{at}dragonet.es.

Journal of Clinical Microbiology, May 2001, p. 1850-1854, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1850-1854.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.