This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nikkari, S.
Right arrow Articles by Relman, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nikkari, S.
Right arrow Articles by Relman, D. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Does Blood of Healthy Subjects Contain Bacterial Ribosomal DNA?

Simo Nikkari,1,2,* Ian J. McLaughlin,3 Wanli Bi,3 Deborah E. Dodge,3,dagger and David A. Relman1,2

Department of Microbiology and Immunology and Department of Medicine, Stanford University School of Medicine, Stanford, California 943051; Veterans Affairs Palo Alto Health Care System, Palo Alto, California 943042; and Applied Biosystems, Foster City, California 944043

Received 24 April 2000/Returned for modification 24 July 2000/Accepted 21 February 2001

Real-time PCR methods with primers and a probe targeting conserved regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger amount of rDNA in blood specimens from healthy individuals than in matched reagent controls. However, the origins and identities of these blood-associated bacterial rDNA sequences remain obscure.

* Corresponding author. Mailing address: PAVAHCS 154T/Relman Lab, Bldg. 101, Rm. B4-175, 3801 Miranda Ave., Palo Alto, CA 94304. Phone: (650) 493-5000, ext. 63163. Fax: (650) 852-3291. E-mail: snikkari{at}cmgm.stanford.edu.

dagger Present address: Bayer Corporation, Berkeley, CA 94702-0466.

Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kumar, S., Wang, L., Fan, J., Kraft, A., Bose, M. E., Tiwari, S., Van Dyke, M., Haigis, R., Luo, T., Ghosh, M., Tang, H., Haghnia, M., Mather, E. L., Weisburg, W. G., Henrickson, K. J. (2008). Detection of 11 Common Viral and Bacterial Pathogens Causing Community-Acquired Pneumonia or Sepsis in Asymptomatic Patients by Using a Multiplex Reverse Transcription-PCR Assay with Manual (Enzyme Hybridization) or Automated (Electronic Microarray) Detection. J. Clin. Microbiol. 46: 3063-3072 [Abstract] [Full Text]  
  • Pingle, M. R., Granger, K., Feinberg, P., Shatsky, R., Sterling, B., Rundell, M., Spitzer, E., Larone, D., Golightly, L., Barany, F. (2007). Multiplexed Identification of Blood-Borne Bacterial Pathogens by Use of a Novel 16S rRNA Gene PCR-Ligase Detection Reaction-Capillary Electrophoresis Assay. J. Clin. Microbiol. 45: 1927-1935 [Abstract] [Full Text]  
  • Perez, P. F., Dore, J., Leclerc, M., Levenez, F., Benyacoub, J., Serrant, P., Segura-Roggero, I., Schiffrin, E. J., Donnet-Hughes, A. (2007). Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?. Pediatrics 119: e724-e732 [Abstract] [Full Text]  
  • Zucol, F., Ammann, R. A., Berger, C., Aebi, C., Altwegg, M., Niggli, F. K., Nadal, D. (2006). Real-Time Quantitative Broad-Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification.. J. Clin. Microbiol. 44: 2750-2759 [Abstract] [Full Text]  
  • Brecher, M. E., Hay, S. N. (2005). Bacterial Contamination of Blood Components. Clin. Microbiol. Rev. 18: 195-204 [Abstract] [Full Text]  
  • Lindholm, L., Sarkkinen, H. (2004). Direct Identification of Gram-Positive Cocci from Routine Blood Cultures by Using AccuProbe Tests. J. Clin. Microbiol. 42: 5609-5613 [Abstract] [Full Text]  
  • Toivanen, P (2003). Normal intestinal microbiota in the aetiopathogenesis of rheumatoid arthritis. Ann Rheum Dis 62: 807-811 [Abstract] [Full Text]  
  • Maaroufi, Y., Heymans, C., De Bruyne, J.-M., Duchateau, V., Rodriguez-Villalobos, H., Aoun, M., Crokaert, F. (2003). Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan-Based PCR Assay. J. Clin. Microbiol. 41: 3293-3298 [Abstract] [Full Text]  
  • McLaughlin, R. W., Vali, H., Lau, P. C. K., Palfree, R. G. E., De Ciccio, A., Sirois, M., Ahmad, D., Villemur, R., Desrosiers, M., Chan, E. C. S. (2002). Are There Naturally Occurring Pleomorphic Bacteria in the Blood of Healthy Humans?. J. Clin. Microbiol. 40: 4771-4775 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»