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Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
Department of Microbiology and Immunology and
Department of Medicine, Stanford University School of Medicine,
Stanford, California 943051; Veterans
Affairs Palo Alto Health Care System, Palo Alto, California
943042; and Applied Biosystems,
Foster City, California 944043
Received 24 April 2000/Returned for modification 24 July
2000/Accepted 21 February 2001
Real-time PCR methods with primers and a probe targeting conserved
regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger
amount of rDNA in blood specimens from healthy individuals than in
matched reagent controls. However, the origins and identities of these
blood-associated bacterial rDNA sequences remain obscure.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Does Blood of Healthy Subjects Contain Bacterial Ribosomal
DNA?
and
*
Corresponding author. Mailing address: PAVAHCS
154T/Relman Lab, Bldg. 101, Rm. B4-175, 3801 Miranda Ave., Palo Alto,
CA 94304. Phone: (650) 493-5000, ext. 63163. Fax: (650) 852-3291. E-mail: snikkari{at}cmgm.stanford.edu.
Present address: Bayer Corporation, Berkeley, CA 94702-0466.
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