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Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Does Blood of Healthy Subjects Contain Bacterial Ribosomal DNA?

Simo Nikkari,1,2,* Ian J. McLaughlin,3 Wanli Bi,3 Deborah E. Dodge,3,dagger and David A. Relman1,2

Department of Microbiology and Immunology and Department of Medicine, Stanford University School of Medicine, Stanford, California 943051; Veterans Affairs Palo Alto Health Care System, Palo Alto, California 943042; and Applied Biosystems, Foster City, California 944043

Received 24 April 2000/Returned for modification 24 July 2000/Accepted 21 February 2001

Real-time PCR methods with primers and a probe targeting conserved regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger amount of rDNA in blood specimens from healthy individuals than in matched reagent controls. However, the origins and identities of these blood-associated bacterial rDNA sequences remain obscure.

* Corresponding author. Mailing address: PAVAHCS 154T/Relman Lab, Bldg. 101, Rm. B4-175, 3801 Miranda Ave., Palo Alto, CA 94304. Phone: (650) 493-5000, ext. 63163. Fax: (650) 852-3291. E-mail: snikkari{at}cmgm.stanford.edu.

dagger Present address: Bayer Corporation, Berkeley, CA 94702-0466.

Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.