Previous Article | Next Article 
Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Does Blood of Healthy Subjects Contain Bacterial Ribosomal
DNA?
Simo
Nikkari,1,2,*
Ian J.
McLaughlin,3
Wanli
Bi,3
Deborah E.
Dodge,3,
and
David A.
Relman1,2
Department of Microbiology and Immunology and
Department of Medicine, Stanford University School of Medicine,
Stanford, California 943051; Veterans
Affairs Palo Alto Health Care System, Palo Alto, California
943042; and Applied Biosystems,
Foster City, California 944043
Received 24 April 2000/Returned for modification 24 July
2000/Accepted 21 February 2001
Real-time PCR methods with primers and a probe targeting conserved
regions of the bacterial 16S ribosomal DNA (rDNA) revealed a larger
amount of rDNA in blood specimens from healthy individuals than in
matched reagent controls. However, the origins and identities of these
blood-associated bacterial rDNA sequences remain obscure.
*
Corresponding author. Mailing address: PAVAHCS
154T/Relman Lab, Bldg. 101, Rm. B4-175, 3801 Miranda Ave., Palo Alto,
CA 94304. Phone: (650) 493-5000, ext. 63163. Fax: (650) 852-3291. E-mail: snikkari{at}cmgm.stanford.edu.

Present address: Bayer Corporation, Berkeley, CA 94702-0466.
Journal of Clinical Microbiology, May 2001, p. 1956-1959, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.1956-1959.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
This article has been cited by other articles:
-
Kumar, S., Wang, L., Fan, J., Kraft, A., Bose, M. E., Tiwari, S., Van Dyke, M., Haigis, R., Luo, T., Ghosh, M., Tang, H., Haghnia, M., Mather, E. L., Weisburg, W. G., Henrickson, K. J.
(2008). Detection of 11 Common Viral and Bacterial Pathogens Causing Community-Acquired Pneumonia or Sepsis in Asymptomatic Patients by Using a Multiplex Reverse Transcription-PCR Assay with Manual (Enzyme Hybridization) or Automated (Electronic Microarray) Detection. J. Clin. Microbiol.
46: 3063-3072
[Abstract]
[Full Text]
-
Pingle, M. R., Granger, K., Feinberg, P., Shatsky, R., Sterling, B., Rundell, M., Spitzer, E., Larone, D., Golightly, L., Barany, F.
(2007). Multiplexed Identification of Blood-Borne Bacterial Pathogens by Use of a Novel 16S rRNA Gene PCR-Ligase Detection Reaction-Capillary Electrophoresis Assay. J. Clin. Microbiol.
45: 1927-1935
[Abstract]
[Full Text]
-
Perez, P. F., Dore, J., Leclerc, M., Levenez, F., Benyacoub, J., Serrant, P., Segura-Roggero, I., Schiffrin, E. J., Donnet-Hughes, A.
(2007). Bacterial Imprinting of the Neonatal Immune System: Lessons From Maternal Cells?. Pediatrics
119: e724-e732
[Abstract]
[Full Text]
-
Zucol, F., Ammann, R. A., Berger, C., Aebi, C., Altwegg, M., Niggli, F. K., Nadal, D.
(2006). Real-Time Quantitative Broad-Range PCR Assay for Detection of the 16S rRNA Gene Followed by Sequencing for Species Identification.. J. Clin. Microbiol.
44: 2750-2759
[Abstract]
[Full Text]
-
Brecher, M. E., Hay, S. N.
(2005). Bacterial Contamination of Blood Components. Clin. Microbiol. Rev.
18: 195-204
[Abstract]
[Full Text]
-
Lindholm, L., Sarkkinen, H.
(2004). Direct Identification of Gram-Positive Cocci from Routine Blood Cultures by Using AccuProbe Tests. J. Clin. Microbiol.
42: 5609-5613
[Abstract]
[Full Text]
-
Toivanen, P
(2003). Normal intestinal microbiota in the aetiopathogenesis of rheumatoid arthritis. Ann Rheum Dis
62: 807-811
[Abstract]
[Full Text]
-
Maaroufi, Y., Heymans, C., De Bruyne, J.-M., Duchateau, V., Rodriguez-Villalobos, H., Aoun, M., Crokaert, F.
(2003). Rapid Detection of Candida albicans in Clinical Blood Samples by Using a TaqMan-Based PCR Assay. J. Clin. Microbiol.
41: 3293-3298
[Abstract]
[Full Text]
-
McLaughlin, R. W., Vali, H., Lau, P. C. K., Palfree, R. G. E., De Ciccio, A., Sirois, M., Ahmad, D., Villemur, R., Desrosiers, M., Chan, E. C. S.
(2002). Are There Naturally Occurring Pleomorphic Bacteria in the Blood of Healthy Humans?. J. Clin. Microbiol.
40: 4771-4775
[Abstract]
[Full Text]