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Journal of Clinical Microbiology, May 2001, p. 1963-1966, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1963-1966.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Real-Time PCR Assay Targeting IS481 of Bordetella pertussis and Molecular Basis for Detecting Bordetella holmesii

Udo Reischl,1,* Norbert Lehn,1 Gary N. Sanden,2 and Mike J. Loeffelholz3

Institute of Medical Microbiology and Hygiene, University of Regensburg, D-93053 Regensburg, Germany1; Division of Bacterial and Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 303332; and State Hygienic Laboratory, University of Iowa, Iowa City, Iowa 522423

Received 13 November 2000/Returned for modification 13 February 2001/Accepted 6 March 2001

Detection of Bordetella holmesii by a real-time PCR assay targeting IS481 of Bordetella pertussis is reported. Sequencing of IS481-specific PCR products from B. pertussis and B. holmesii isolates revealed sequence homology. Restriction fragment length polymorphism demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B. holmesii from patients with cough, suggest that the specificity and predictive value of IS481-based PCR assays for pertussis may be compromised.

* Corresponding author. Mailing address: Institut für Medizinische Mikrobiologie und Hygiene, Universitätsklinikum Regensburg, Franz-Josef-Strauß-Allee 11, D-93053 Regensburg, Germany. Phone: 49-941-59609-50. Fax: 49-941-944-6402. E-mail: Udo.Reischl{at}klinik.uni-regensburg.de.

Journal of Clinical Microbiology, May 2001, p. 1963-1966, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1963-1966.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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