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Journal of Clinical Microbiology, May 2001, p. 1963-1966, Vol. 39, No. 5
Institute of Medical Microbiology and
Hygiene, University of Regensburg, D-93053 Regensburg,
Germany1; Division of Bacterial and
Mycotic Diseases, Centers for Disease Control and Prevention, Atlanta,
Georgia 303332; and State Hygienic
Laboratory, University of Iowa, Iowa City, Iowa
522423
Received 13 November 2000/Returned for modification 13 February
2001/Accepted 6 March 2001
Detection of Bordetella holmesii by a real-time PCR
assay targeting IS481 of Bordetella
pertussis is reported. Sequencing of
IS481-specific PCR products from B.
pertussis and B. holmesii isolates revealed
sequence homology. Restriction fragment length polymorphism
demonstrated a low copy number of IS481-like sequences in B. holmesii. These results, and culture of B.
holmesii from patients with cough, suggest that the specificity
and predictive value of IS481-based PCR assays for
pertussis may be compromised.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.1963-1966.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Real-Time PCR Assay Targeting IS481
of Bordetella pertussis and Molecular Basis for
Detecting Bordetella holmesii
*
Corresponding author. Mailing address: Institut
für Medizinische Mikrobiologie und Hygiene,
Universitätsklinikum Regensburg, Franz-Josef-Strauß-Allee
11, D-93053 Regensburg, Germany. Phone: 49-941-59609-50. Fax:
49-941-944-6402. E-mail:
Udo.Reischl{at}klinik.uni-regensburg.de.
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