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Journal of Clinical Microbiology, May 2001, p. 1967-1968, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1967-1968.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Pretreatment with Urea-Hydrochloric Acid Enhances the Isolation of Helicobacter pylori from Contaminated Specimens

Qunsheng Song,1,2 Gerald W. Zirnstein,1 Bala Swaminathan,1 and Benjamin D. Gold1,2,*

Foodborne and Diarrheal Diseases Branch, Centers for Disease Control and Prevention, Atlanta, Georgia 30333,1 and Division of Pediatric Gastroenterology and Nutrition, Department of Pediatrics, Emory University School of Medicine, Atlanta, Georgia 303222

Received 4 October 2000/Returned for modification 2 January 2001/Accepted 1 March 2001

Human saliva seeded with H. pylori was incubated in urea-HCl and then cultured on nonselective media. Pretreatment with 0.06 N HCl-0.08 M urea for 5 min at 37°C resulted in reproducible isolation of H. pylori, even at low inocula (<= 102 CFU/ml of saliva), despite the presence of large numbers of contaminating organisms.

* Corresponding author. Mailing address: Division of Gastroenterology and Nutrition, Department of Pediatrics, Emory University School of Medicine, 2040 Ridgewood Dr., NE, Atlanta, GA 30322. Phone: (404) 727-1463. Fax: (404) 727-2120. E-mail: ben_gold{at}oz.ped.emory.edu.

Journal of Clinical Microbiology, May 2001, p. 1967-1968, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1967-1968.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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