This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hanson, K. L.
Right arrow Articles by Cartwright, C. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hanson, K. L.
Right arrow Articles by Cartwright, C. P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, May 2001, p. 1975-1977, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1975-1977.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of an Automated Liquid-Handling System (Tecan Genesis RSP 100) in the Abbott LCx Assay for Chlamydia trachomatis

Kevan L. Hanson1 and Charles P. Cartwright1,2,*

Department of Laboratory Medicine and Pathology, Hennepin County Medical Center, Minneapolis, Minnesota 55415,1 and Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Minneapolis, Minnesota 554552

Received 27 November 2000/Returned for modification 14 January 2001/Accepted 18 February 2001

The present study investigated the feasibility of automating the specimen-pipetting component of sample preparation in the LCx Chlamydia assay (LCx-CT assay; Abbott Laboratories, Chicago, Ill.) by using a commercially available liquid-handling system (Tecan Genesis RSP100; Tecan Inc., Research Triangle Park, N.C.). The Tecan instrument proved to be comparable in both precision and accuracy to a manual multipipettor (Eppendorf model 4850; Eppendorf Scientific, Westbury, N.Y.). The Tecan instrument was extensively checked for evidence of specimen-to-specimen transfer, and no level of contamination sufficient to generate a signal above the background in the LCx-CT assay was detected. Finally, pipetting speed was significantly improved by using the Tecan instrument. A mean time of 2.5 min was required to pipette a complete LCx-CT assay carousel (20 samples and 4 controls) with the Tecan instrument, whereas 8.4 min was required to pipette a comparable number of samples manually (P < 0.001).

* Corresponding author. Mailing address: Clinical Laboratories, MC #812, Hennepin County Medical Center, 701 Park Ave., Minneapolis, MN 55415. Phone: (612) 347-3026. Fax: (612) 904-4229. E-mail: charles.cartwright{at}co.hennepin.mn.us.

Journal of Clinical Microbiology, May 2001, p. 1975-1977, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.1975-1977.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Marshall, R., Chernesky, M., Jang, D., Hook, E. W., Cartwright, C. P., Howell-Adams, B., Ho, S., Welk, J., Lai-Zhang, J., Brashear, J., Diedrich, B., Otis, K., Webb, E., Robinson, J., Yu, H. (2007). Characteristics of the m2000 Automated Sample Preparation and Multiplex Real-Time PCR System for Detection of Chlamydia trachomatis and Neisseria gonorrhoeae. J. Clin. Microbiol. 45: 747-751 [Abstract] [Full Text]  
  • Chernesky, M A (2002). Chlamydia trachomatis diagnostics. Sex. Transm. Infect. 78: 232-234 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»