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Journal of Clinical Microbiology, May 2001, p. 2002-2005, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2002-2005.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Actinobacillus actinomycetemcomitans Serotypes by Multiplex PCR

Nao Suzuki, Yoshio Nakano,* Yasuo Yoshida, Daisuke Ikeda, and Toshihiko Koga

Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan

Received 23 October 2000/Returned for modification 4 January 2001/Accepted 5 March 2001

Oligonucleotide primers specific for gene clusters involved in the biosynthesis of serotype-specific polysaccharide antigens were designed to identify Actinobacillus actinomycetemcomitans serotypes a to e using the multiplex PCR. This method may be useful for serotype-specific genotyping rapidly and directly from clinical samples containing various organisms.

* Corresponding author. Mailing address: Department of Preventive Dentistry, Kyushu University Faculty of Dental Science, Fukuoka 812-8582, Japan. Phone: 81 92 642 6423. Fax: 81 92 642 6354. E-mail: yosh{at}dent.kyushu-u.ac.jp.

Journal of Clinical Microbiology, May 2001, p. 2002-2005, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2002-2005.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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