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Journal of Clinical Microbiology, May 2001, p. 2002-2005, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.2002-2005.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Identification of Actinobacillus
actinomycetemcomitans Serotypes by Multiplex PCR
Nao
Suzuki,
Yoshio
Nakano,*
Yasuo
Yoshida,
Daisuke
Ikeda, and
Toshihiko
Koga
Department of Preventive Dentistry, Kyushu
University Faculty of Dental Science, Fukuoka 812-8582, Japan
Received 23 October 2000/Returned for modification 4 January
2001/Accepted 5 March 2001
Oligonucleotide primers specific for gene clusters involved in the
biosynthesis of serotype-specific polysaccharide antigens were designed
to identify Actinobacillus actinomycetemcomitans serotypes
a to e using the multiplex PCR. This method may be useful for
serotype-specific genotyping rapidly and directly from clinical samples
containing various organisms.
*
Corresponding author. Mailing address: Department of
Preventive Dentistry, Kyushu University Faculty of Dental Science,
Fukuoka 812-8582, Japan. Phone: 81 92 642 6423. Fax: 81 92 642 6354. E-mail: yosh{at}dent.kyushu-u.ac.jp.
Journal of Clinical Microbiology, May 2001, p. 2002-2005, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.2002-2005.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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