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Journal of Clinical Microbiology, May 2001, p. 2006-2008, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2006-2008.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of PCR Using TRC4 and IS6110 Primers in Detection of Tuberculous Meningitis

Sujatha Narayanan,1,* Vijayalakshmi Parandaman,1 P. R. Narayanan,1 P. Venkatesan,1 C. Girish,2 S. Mahadevan,2 and Sarala Rajajee3

Tuberculosis Research Centre, Chetput, Chennai 600 031,1 Department of Pediatrics, JIPMER, Pondicherry 605 006,2 and Childs Trust Hospital, Chennai 600 034,3 India

Received 15 September 2000/Returned for modification 1 December 2000/Accepted 7 March 2001

We have evaluated a new set of primers (TRC4) in comparison with the IS6110 primers commonly used in PCR to detect tuberculous meningitis among children. The levels of concordance between the results of IS6110 PCR and TRC4 PCR with cerebrospinal fluid specimens from patients with clinically confirmed tuberculous meningitis were 80 and 86%, respectively. Results with the two primer sets were concordant for 55 positive and 22 negative specimens (n = 98). We conclude that the sensitivity of PCR can be increased by using both IS6110 and TRC4 primers.

* Corresponding author. Mailing address: Tuberculosis Research Centre, Mayor V. R. Ramanathan Rd., Chetput, Chennai 600 031, India. Phone: 91-(044)-8265425. Fax: 91-(044)-8262137. E-mail: Sujatha36{at}hotmail.com.

Journal of Clinical Microbiology, May 2001, p. 2006-2008, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2006-2008.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.