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Journal of Clinical Microbiology, May 2001, p. 2015-2016, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2015-2016.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Evaluation of a Reformulated CHROMagar Candida

Mary Ann Jabra-Rizk,1,2,* Troy M. Brenner,3 Mark Romagnoli,3 A. A. M. A. Baqui,1 William G. Merz,3 William A. Falkler Jr.,2 and Timothy F. Meiller1

Department of Oral Medicine1 and Department of OCBS,2 Dental School, University of Maryland, and Department of Pathology, The Johns Hopkins University,3 Baltimore, Maryland

Received 11 August 2000/Returned for modification 29 November 2000/Accepted 6 March 2001

CHROMagar Candida is a differential culture medium for the isolation and presumptive identification of clinically important yeasts. Recently the medium was reformulated by Becton Dickinson. This study was designed to evaluate the performance of the new formula of CHROMagar against the original CHROMagar Candida for recovery, growth, and colony color with stock cultures and with direct plating of clinical specimens. A total of 90 stock yeast isolates representing nine yeast species, including Candida dubliniensis, as well as 522 clinical specimens were included in this study. No major differences were noted in growth rate or colony size between the two media for most of the species. However, all 10 Candida albicans isolates evaluated consistently gave a lighter shade of green on the new CHROMagar formulation. In contrast, all 26 C. dubliniensis isolates gave the same typical dark green color on both media. A total of 173 of the 522 clinical specimens were positive for yeast, with eight yeast species recovered. The recovery rates for each species were equivalent on both media, with no consistent species-associated differences in colony size or color. Although both media were comparable in performance, the lighter green colonies of C. albicans isolates on the new CHROMagar made it easier to differentiate between C. albicans and C. dubliniensis isolates. In conclusion, the newly formulated Becton Dickinson CHROMagar Candida medium is as equally suited as a differential medium for the presumptive identification of yeast species and for the detection of multiple yeast species in clinical specimens as the original CHROMagar Candida medium.

* Corresponding author. Mailing address: Department of Oral Medicine, Dental School, UMAB, 666 W. Baltimore St., Baltimore, MD 21201. Phone: (410) 708-7628. Fax: (410) 706-0519. E-mail: mrizk{at}umaryland.edu.

Journal of Clinical Microbiology, May 2001, p. 2015-2016, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2015-2016.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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