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Journal of Clinical Microbiology, May 2001, p. 2015-2016, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.2015-2016.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Evaluation of a Reformulated CHROMagar
Candida
Mary Ann
Jabra-Rizk,1,2,*
Troy M.
Brenner,3
Mark
Romagnoli,3
A. A. M. A.
Baqui,1
William G.
Merz,3
William A.
Falkler Jr.,2 and
Timothy F.
Meiller1
Department of Oral
Medicine1 and Department of
OCBS,2 Dental School, University of
Maryland, and Department of Pathology, The Johns Hopkins
University,3 Baltimore, Maryland
Received 11 August 2000/Returned for modification 29 November
2000/Accepted 6 March 2001
CHROMagar Candida is a differential culture medium for the
isolation and presumptive identification of clinically important yeasts. Recently the medium was reformulated by Becton Dickinson. This
study was designed to evaluate the performance of the new formula of
CHROMagar against the original CHROMagar Candida for recovery, growth,
and colony color with stock cultures and with direct plating of
clinical specimens. A total of 90 stock yeast isolates representing
nine yeast species, including Candida dubliniensis, as well
as 522 clinical specimens were included in this study. No major
differences were noted in growth rate or colony size between the two
media for most of the species. However, all 10 Candida
albicans isolates evaluated consistently gave a lighter shade of
green on the new CHROMagar formulation. In contrast, all 26 C. dubliniensis isolates gave the same typical dark green color on
both media. A total of 173 of the 522 clinical specimens were positive
for yeast, with eight yeast species recovered. The recovery rates for
each species were equivalent on both media, with no consistent
species-associated differences in colony size or color. Although both
media were comparable in performance, the lighter green colonies of
C. albicans isolates on the new CHROMagar made it easier to
differentiate between C. albicans and C. dubliniensis isolates. In conclusion, the newly formulated Becton
Dickinson CHROMagar Candida medium is as equally suited as a
differential medium for the presumptive identification of yeast species
and for the detection of multiple yeast species in clinical specimens
as the original CHROMagar Candida medium.
*
Corresponding author. Mailing address: Department of
Oral Medicine, Dental School, UMAB, 666 W. Baltimore St., Baltimore, MD
21201. Phone: (410) 708-7628. Fax: (410) 706-0519. E-mail: mrizk{at}umaryland.edu.
Journal of Clinical Microbiology, May 2001, p. 2015-2016, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.2015-2016.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
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