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Journal of Clinical Microbiology, May 2001, p. 2017-2021, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2017-2021.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep

Steven P. Djordjevic,1,* Michael A. Hornitzky,1 Graham Bailey,2 Paul Gill,3 Barbara Vanselow,4 Keith Walker,1 and Karl A. Bettelheim5

Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, Camden, New South Wales 2570,1 Orange Regional Veterinary Laboratory, Orange, New South Wales 2800,2 Wollongbar Regional Veterinary Laboratory, Wollongbar, New South Wales 2477,3 NSW Agriculture Beef Industry Centre, UNE, Armidale, New South Wales 2351,4 and Microbiological Diagnostic Unit, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052,5 Australia

Received 21 September 2000/Returned for modification 9 January 2001/Accepted 8 March 2001

A group of 1,623 ovine fecal samples recovered from 65 geographically distinct mutton sheep and prime lamb properties across New South Wales, Australia, were screened for the presence of Shiga toxin-producing Escherichia coli (STEC) virulence factors (stx1, stx2, eaeA, and ehxA). A subset was cultured for STEC isolates containing associated virulence factors (eaeA and/or ehxA), which were isolated from 17 of 20 (85%) and 19 of 20 (95%) tested prime lamb and mutton sheep properties, respectively. STEC isolates containing stx1, stx2, and ehxA were most commonly isolated (19 of 40 flocks; 47.5%), and this profile was observed for 10 different serotypes. Among 90 STEC isolates studied, the most common serotypes were O91:H- (22 isolates [24.4%]), O5:H- (16 isolates [17.8%]), O128:H2 (11 isolates [12.2%]), O123:H- (8 isolates [8.9%]), and O85:H49 (5 isolates [5.6%]). Two isolates (2.2%) were typed as O157:H-. A total of 78 of 90 STEC isolates (86.7%) expressed Shiga toxin in Vero cell culture and 75 of 84 ehxA-positive isolates (89.3%) expressed enterohemolysin on washed sheep blood agar. eaeA was observed in 11 of 90 (12.2%) ovine STEC isolates, including serotypes O5:H-, O84:H-, O85:H49, O123:H- O136:H40, and O157:H-. Although only 2 of 90 isolates were typed as O157:H-, the predominant serotypes recovered during this study have been recovered from human patients with clinical disease, albeit rarely.

* Corresponding author. Mailing address: Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, Private Mail Bag 8, Camden, New South Wales 2570, Australia. Phone: 0061-246-406426. Fax: 0061-246-406384. E-mail: steve.djordjevic{at}agric.nsw.gov.au.

Journal of Clinical Microbiology, May 2001, p. 2017-2021, Vol. 39, No. 5
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.5.2017-2021.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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