Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep

doi:10.1128/JCM.39.5.2017-2021.2001

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Journal of Clinical Microbiology, May 2001, p. 2017-2021, Vol. 39, No. 5
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.5.2017-2021.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep

Steven P. Djordjevic,¹^,^* Michael A. Hornitzky,¹ Graham Bailey,² Paul Gill,³ Barbara Vanselow,⁴ Keith Walker,¹ and Karl A. Bettelheim⁵

Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, Camden, New South Wales 2570,¹ Orange Regional Veterinary Laboratory, Orange, New South Wales 2800,² Wollongbar Regional Veterinary Laboratory, Wollongbar, New South Wales 2477,³ NSW Agriculture Beef Industry Centre, UNE, Armidale, New South Wales 2351,⁴ and Microbiological Diagnostic Unit, Department of Microbiology and Immunology, University of Melbourne, Parkville, Victoria 3052,⁵ Australia

Received 21 September 2000/Returned for modification 9 January 2001/Accepted 8 March 2001

A group of 1,623 ovine fecal samples recovered from 65 geographically distinct mutton sheep and prime lamb properties acrossNew South Wales, Australia, were screened for the presence ofShiga toxin-producing Escherichia coli (STEC) virulence factors(stx₁, stx₂, eaeA, and ehxA). A subset was cultured for STEC isolatescontaining associated virulence factors (eaeA and/or ehxA), whichwere isolated from 17 of 20 (85%) and 19 of 20 (95%) tested primelamb and mutton sheep properties, respectively. STEC isolatescontaining stx₁, stx₂, and ehxA were most commonly isolated (19of 40 flocks; 47.5%), and this profile was observed for 10 differentserotypes. Among 90 STEC isolates studied, the most common serotypeswere O91:H (22 isolates [24.4%]), O5:H (16 isolates [17.8%]), O128:H2 (11 isolates [12.2%]), O123:H (8 isolates [8.9%]), and O85:H49 (5 isolates [5.6%]). Two isolates(2.2%) were typed as O157:H. A total of 78 of 90 STEC isolates (86.7%) expressed Shiga toxinin Vero cell culture and 75 of 84 ehxA-positive isolates (89.3%)expressed enterohemolysin on washed sheep blood agar. eaeA wasobserved in 11 of 90 (12.2%) ovine STEC isolates, including serotypesO5:H, O84:H, O85:H49, O123:H O136:H40, and O157:H. Although only 2 of 90 isolates were typed as O157:H, the predominant serotypes recovered during this study have beenrecovered from human patients with clinical disease, albeitrarely.

^* Corresponding author. Mailing address: Elizabeth Macarthur Agricultural Institute, New South Wales Agriculture, Private MailBag 8, Camden, New South Wales 2570, Australia. Phone: 0061-246-406426.Fax: 0061-246-406384. E-mail: steve.djordjevic{at}agric.nsw.gov.au.

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