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Journal of Clinical Microbiology, June 2001, p. 2055-2059, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2055-2059.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Quantitation of Human Immunodeficiency Virus Type 1 (HIV-1) RNA in Cell-Free Cervicovaginal Secretions: Comparison of Reverse Transcription-PCR Amplification (AMPLICOR HIV-1 MONITOR 1.5) with Enhanced-Sensitivity Branched-DNA Assay (Quantiplex 3.0)

Ali Si-Mohamed,1,2,* Laurent Andreoletti,1,2 Isabelle Colombet,3 Marie-Paule Carreno,2 Gladys Lopez,4 Gilles Chatelier,3 Michel D. Kazatchkine,2 and Laurent Belec1,2

Laboratoire de Virologie, Hôpital Européen Georges Pompidou,1 INSERM Unité 430 and Université Pierre et Marie Curie (Université Paris VI), Hôpital Broussais,2 and Département de Statistiques, Université Pierre et Marie Curie,3 Paris, France, and Laboratorio del Programa Nacional de Lucha contra el SIDA, Asuncion, Paraguay4

Received 20 November 2000/Returned for modification 10 January 2001/Accepted 12 March 2001

Two commercially available hypersensitive assays for human immunodeficiency virus type 1 (HIV-1) RNA quantitation, AMPLICOR HIV-1 Monitor Test 1.5 and Quantiplex HIV RNA 3.0, were compared to detect and quantify HIV-1 RNA in the cell-free fraction of cervicovaginal secretions collected by vaginal washing. Three panel specimens were used: pooled cervicovaginal secretions spiked with HIV-1 subtype A or HIV-1 subtype B and cervicovaginal lavages from HIV-positive and HIV-negative women. Compared to the AMPLICOR HIV-1 Monitor Test 1.5 assay, the Quantiplex HIV-1 3.0 assay yielded higher estimates of HIV-1 RNA concentrations in several tested samples spiked with HIV-1 RNA subtype A, as well as subtype B, particularly samples containing low amounts of HIV-1 RNA. The sensitivity and specificity of the AMPLICOR HIV-1 Monitor Test 1.5 assay were 93 and 100%, respectively; the sensitivity and specificity of the Quantiplex HIV RNA 3.0 assay were 97 and 50%, respectively. In conclusion, in quantifying HIV-1 RNA in cervicovaginal secretions, the Quantiplex HIV RNA 3.0 may lack specificity, and the AMPLICOR HIV-1 Monitor Test 1.5 assay, although highly specific, may lack sensitivity.

* Corresponding author. Mailing address: Laboratoire de Virologie, Service de Microbiologie, Hôpital Européen Georges Pompidou, 20, rue Leblanc, 75 908 Paris Cedex 15, France. Phone: (331) 56-09-39-58. Fax: (331) 56-09-24-47. E-mail: ali.simohamed{at}egp.ap-hop-paris.fr.

Journal of Clinical Microbiology, June 2001, p. 2055-2059, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2055-2059.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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