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Journal of Clinical Microbiology, June 2001, p. 2122-2125, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2122-2125.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Simple and Efficient Method for Measuring Anti-Toxoplasma Immunoglobulin Antibodies in Human Sera Using Complement-Mediated Lysis of Transgenic Tachyzoites Expressing beta -Galactosidase

Caroline Dando,1,2,dagger Katie E. Gabriel,1 Jack S. Remington,1,2,* and Stephen F. Parmley1,Dagger

Department of Immunology and Infectious Diseases, Research Institute, 795 El Camino Real, Palo Alto, California 94301,1 and Division of Infectious Diseases, Stanford University School of Medicine, Stanford, California 943052

Received 10 October 2000/Returned for modification 16 November 2000/Accepted 6 March 2001

A simple and efficient method using transgenic Toxoplasma gondii tachyzoites expressing beta -galactosidase was developed for detection of specific antibodies against the parasite in sera of patients. The titers obtained with the new test were similar to those obtained with the Sabin-Feldman dye test run in parallel. Although significant changes in endpoint titers were not observed when sera drawn sequentially at 2- to 3-week intervals were tested with both procedures, apparent differences in antibody affinity were observed with the new test which were not perceptible with the Sabin-Feldman dye test. Like the Sabin-Feldman dye test, the new test is based on complement lysis of tachyzoites, but it is much easier to perform and the reaction is read colorimetrically instead of visually.

* Corresponding author. Mailing address: Research Institute, 795 El Camino Real, Palo Alto, CA 94301. Phone: (650) 853-6061. Fax: (650) 329-9853. E-mail: remington{at}pamf.org.

dagger Present address: Lynx Therapeutics, Hayward, CA 94545.

Dagger Present address: Maxygen, Redwood City, CA 94063.

Journal of Clinical Microbiology, June 2001, p. 2122-2125, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2122-2125.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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