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Journal of Clinical Microbiology, June 2001, p. 2248-2253, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2248-2253.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genotyping Encephalitozoon cuniculi by Multilocus Analyses of Genes with Repetitive Sequences

Lihua Xiao,1,* Lixia Li,1 Govinda S. Visvesvara,1 Hercules Moura,1 Elizabeth S. Didier,2 and Altaf A. Lal1

Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Public Health Service, U.S. Department of Health and Human Services, Atlanta, Georgia 30341,1 and Department of Microbiology, Tulane Regional Primate Research Center, Tulane University Medical Center, Covington, Louisiana 704332

Received 29 January 2001/Returned for modification 22 March 2001/Accepted 8 April 2001

Encephalitozoon cuniculi infects a wide range of mammalian hosts. Three genotypes based on the number of GTTT repeats in the internal transcribed spacer (ITS) of the rRNA have been described, of which genotypes I and III have been identified in humans. In this study, the genetic diversity of E. cuniculi was examined at the polar tube protein (PTP) and spore wall protein I (SWP-1) loci. Nucleotide sequence analysis of the PTP gene divided 11 E. cuniculi isolates into three genotypes in congruence with the result of analysis of the ITS of the rRNA gene. The three PTP genotypes differed from one another by the copy number of the 78-bp central repeat as well as point mutations. These E. cuniculi isolates also differed from one another in the number of 15- and 36-bp repeats in the SWP-1 gene. In addition, some E. cuniculi isolates had heterogeneous copies of the SWP-1 gene with various numbers of repeats. Intragenotypic variation was also observed at the SWP-1 locus. Based on the length polymorphism and sequence diversities of the PTP and SWP-1 genes, two simple PCR tests were developed to differentiate E. cuniculi in clinical samples.

* Corresponding author. Mailing address: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770, Buford Highway, Atlanta, GA 30341. Phone: (770) 488-4840. Fax: (770) 488-4454. E-mail: LAX0{at}CDC.GOV.

Journal of Clinical Microbiology, June 2001, p. 2248-2253, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2248-2253.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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