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Journal of Clinical Microbiology, June 2001, p. 2283-2286, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2283-2286.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparative Study Using Amplified Fragment Length Polymorphism Fingerprinting, PCR Genotyping, and Phenotyping To Differentiate Campylobacter fetus Strains Isolated from Animals

Jaap A. Wagenaar,1,* Marcel A. P. van Bergen,1 Diane G. Newell,2 Rose Grogono-Thomas,2,dagger and Birgitta Duim1

Institute for Animal Science and Health, Lelystad, The Netherlands,1 and Veterinary Laboratories Agency (Weybridge), New Haw, United Kingdom2

Received 28 August 2000/Returned for modification 30 November 2000/Accepted 26 March 2001

A collection of Campylobacter fetus strains, including both C. fetus subsp. fetus and C. fetus subsp. venerealis, were phenotypically identified to the subspecies level and genotypically typed by PCR and amplified fragment length polymorphism (AFLP) analysis. Phenotypic subspecies determination methods were unreliable. Genotyping of the strains by PCR and AFLP showed a clear discrimination between the two subspecies.

* Corresponding author. Mailing address: Institute for Animal Science and Health, P.O. Box 65, 8200 AB Lelystad, The Netherlands. Phone: 31-320-238157. Fax: 31 320-238153. E-mail: j.a.wagenaar{at}id.wag-ur.nl.

dagger Present address: Department of Clinical Veterinary Science, Division of Animal Health and Husbandry, Bristol, United Kingdom.

Journal of Clinical Microbiology, June 2001, p. 2283-2286, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2283-2286.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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