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Journal of Clinical Microbiology, June 2001, p. 2300-2305, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2300-2305.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Prevalence of Iron Transport Gene on Pathogenicity-Associated Island of Uropathogenic Escherichia coli in E. coli O157:H7 Containing Shiga Toxin Gene

Changyun Ye and Jianguo Xu*

Priority Laboratory of Medical Molecular Bacteriology of the Ministry of Health, Institute of Epidemiology and Microbiology, Chinese Academy of Preventive Medicine, Changping, Beijing 102206, People's Republic of China

Received 31 October 2000/Returned for modification 13 December 2000/Accepted 14 March 2001

Uropathogenc Escherichia coli (UPEC) CFT073 has a pathogenicity-associated island (PAICFT073), which causes pyelonephritis and cystitis. Using PCR method, we found the prrA gene of PAICFT073 in E. coli O157:H7 EDL933. Further detailed PCR screening of 38 open reading frames, the right and left junction sequences of PAICFT073, revealed that it is the prrA-modD-yc73-fepC gene cluster but not the PAICFT073 present in E. coli O157:H7 EDL933. A rapid preliminary analysis suggested that the prrA-modD-yc73-fepC gene cluster of the PAICFT073, is present in 43 strains of E. coli O157:H7 containing Shiga toxin (Stx) gene but absent in 19 strains of E. coli O157:H7 without Stx gene. A strict co-occurrence of the prrA-modD-yc73-fepC gene cluster and Stx genes was observed, regardless of their origin. The prrA-modD-yc73-fepC gene cluster encode proteins probably involved in iron uptake system, which strongly suggests the importance of iron metabolism in the Stx-mediated virulence. In addition, the prrA-modD-yc73-fepC gene cluster may be used as a diagnostic marker to distinguish E. coli O157:H7 strains containing Stx gene from that without Stx gene, and possibly to quickly detect other pathogenic gram-negative bacteria containing the Stx gene.

* Corresponding author. Mailing address: Institute of Epidemiology and Microbiology, Chinese Academy for Preventive Medicine, P.O. Box 5, Changpin, Beijing 102206, People's Republic of China. Phone: 86-10-61739579. Fax: 86-10-61730233. E-mail: xujg{at}public.bta.net.cn.

Journal of Clinical Microbiology, June 2001, p. 2300-2305, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2300-2305.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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