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Journal of Clinical Microbiology, June 2001, p. 2325-2329, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2325-2329.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Genotyping Neisseria gonorrhoeae Using Fluorescent Amplified Fragment Length Polymorphism Analysis

Helen M. Palmer1 and Catherine Arnold2,*

Genitourinary Infections Reference Laboratory, Public Health Laboratory, Bristol Royal Infirmary, Bristol BS2 8HW,1 and SBVL, Central Public Health Laboratory, London NW9 5HT,2 United Kingdom

Received 18 January 2001/Returned for modification 4 March 2001/Accepted 31 March 2001

An evaluation of fluorescent amplified fragment length polymorphism (FAFLP) for typing Neisseria gonorrhoeae using 52 isolates revealed that its discriminatory power approached that of Opa-typing in identifying epidemiologically linked isolates. Automated, accurate sizing of FAFLP amplified fragments permits objective data analysis and storage, making it an attractive method for large surveillance projects.

* Corresponding author. Mailing address: SBVL, Central Public Health Laboratory, 61 Colindale Ave., London NW9 5HT, United Kingdom. Phone: 44 20 8200 4400. Fax: 44 20 8200 1569. E-mail: carnold{at}phls.nhs.uk.

Journal of Clinical Microbiology, June 2001, p. 2325-2329, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2325-2329.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.




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