This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hayette, M.-P.
Right arrow Articles by De Mol, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hayette, M.-P.
Right arrow Articles by De Mol, P.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2001, p. 2338-2340, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2338-2340.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Aspergillus Species DNA by PCR in Bronchoalveolar Lavage Fluid

Marie-Pierre Hayette,1,* Dolores Vaira,2 Fabrice Susin,1 Pascal Boland,1 Geneviève Christiaens,1 Pierrette Melin,1 and Patrick De Mol1

Service de Microbiologie Médicale1 and Service de Transfusion,2 Tour de Pathologie B23, Université de Liège, B-4000 Liège, Belgium

Received 20 November 2000/Returned for modification 7 February 2001/Accepted 22 March 2001

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization.

* Corresponding author. Mailing address: Service de Microbiologie Médicale, Tour de Pathologie B23, Université de Liège, B-4000 Liège, Belgium. Phone: 32/43.66.24.36. Fax: 32/43.66.24.40. E-mail: mphayette{at}ulg.ac.be.

Journal of Clinical Microbiology, June 2001, p. 2338-2340, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2338-2340.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Bulpa, P., Dive, A., Sibille, Y. (2007). Invasive pulmonary aspergillosis in patients with chronic obstructive pulmonary disease. Eur Respir J 30: 782-800 [Abstract] [Full Text]  
  • Musher, B., Fredricks, D., Leisenring, W., Balajee, S. A., Smith, C., Marr, K. A. (2004). Aspergillus Galactomannan Enzyme Immunoassay and Quantitative PCR for Diagnosis of Invasive Aspergillosis with Bronchoalveolar Lavage Fluid. J. Clin. Microbiol. 42: 5517-5522 [Abstract] [Full Text]  
  • de Aguirre, L., Hurst, S. F., Choi, J. S., Shin, J. H., Hinrikson, H. P., Morrison, C. J. (2004). Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR-Enzyme Immunoassay. J. Clin. Microbiol. 42: 3495-3504 [Abstract] [Full Text]  
  • O'Sullivan, C. E., Kasai, M., Francesconi, A., Petraitis, V., Petraitiene, R., Kelaher, A. M., Sarafandi, A. A., Walsh, T. J. (2003). Development and Validation of a Quantitative Real-Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 41: 5676-5682 [Abstract] [Full Text]  
  • Rantakokko-Jalava, K., Laaksonen, S., Issakainen, J., Vauras, J., Nikoskelainen, J., Viljanen, M. K., Salonen, J. (2003). Semiquantitative Detection by Real-Time PCR of Aspergillus fumigatus in Bronchoalveolar Lavage Fluids and Tissue Biopsy Specimens from Patients with Invasive Aspergillosis. J. Clin. Microbiol. 41: 4304-4311 [Abstract] [Full Text]  
  • Sanguinetti, M., Posteraro, B., Pagano, L., Pagliari, G., Fianchi, L., Mele, L., La Sorda, M., Franco, A., Fadda, G. (2003). Comparison of Real-Time PCR, Conventional PCR, and Galactomannan Antigen Detection by Enzyme-Linked Immunosorbent Assay Using Bronchoalveolar Lavage Fluid Samples from Hematology Patients for Diagnosis of Invasive Pulmonary Aspergillosis. J. Clin. Microbiol. 41: 3922-3925 [Abstract] [Full Text]  
  • Spiess, B., Buchheidt, D., Baust, C., Skladny, H., Seifarth, W., Zeilfelder, U., Leib-Mosch, C., Morz, H., Hehlmann, R. (2003). Development of a LightCycler PCR Assay for Detection and Quantification of Aspergillus fumigatus DNA in Clinical Samples from Neutropenic Patients. J. Clin. Microbiol. 41: 1811-1818 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»