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Journal of Clinical Microbiology, June 2001, p. 2338-2340, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2338-2340.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of Aspergillus Species DNA by PCR in Bronchoalveolar Lavage Fluid

Marie-Pierre Hayette,1,* Dolores Vaira,2 Fabrice Susin,1 Pascal Boland,1 Geneviève Christiaens,1 Pierrette Melin,1 and Patrick De Mol1

Service de Microbiologie Médicale1 and Service de Transfusion,2 Tour de Pathologie B23, Université de Liège, B-4000 Liège, Belgium

Received 20 November 2000/Returned for modification 7 February 2001/Accepted 22 March 2001

The usefulness of a nested PCR assay for detection of Aspergillus sp. DNA was evaluated in 177 bronchoalveolar lavage (BAL) fluid specimens. This test was accurate both to diagnose culture-negative BAL fluid specimens from patients with invasive pulmonary aspergillosis and to confirm culture-positive samples. However, it did not differentiate between infection and colonization.


* Corresponding author. Mailing address: Service de Microbiologie Médicale, Tour de Pathologie B23, Université de Liège, B-4000 Liège, Belgium. Phone: 32/43.66.24.36. Fax: 32/43.66.24.40. E-mail: mphayette{at}ulg.ac.be.


Journal of Clinical Microbiology, June 2001, p. 2338-2340, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2338-2340.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.



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Copyright © 2001 by the American Society for Microbiology. All rights reserved.