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Journal of Clinical Microbiology, June 2001, p. 2344-2347, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2344-2347.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sampling Variability in the Microbiological Evaluation of Expectorated Sputa and Endotracheal Aspirates

Sanjai Nagendra,1 Paul Bourbeau,2 Stephen Brecher,3 Michael Dunne,4 Mark LaRocco,5 and Gary Doern1,*

University of Iowa College of Medicine, Iowa City, Iowa1; Geisinger Medical Center, Danville, Pennsylvania2; Veterans Administration Medical Center, Boston, Massachusetts3; Henry Ford Health System, Detroit, Michigan4; and St. Lukes Episcopal Medical Center, Houston, Texas5

Received 1 November 2000/Returned for modification 2 March 2001/Accepted 20 March 2001

A five-center study was conducted with the aim of determining how reproducibly expectorated sputa and tracheal aspirates could be sampled when preparing Gram-stained smears and inoculating cultures. With both specimen types, excessive variation was noted among Gram stain results obtained from replicate smears. Less variation was noted among culture results, especially with tracheal aspirates.

* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 356-8616. Fax: (319) 356-4916. E-mail: gary-doern{at}uiowa.edu.

Journal of Clinical Microbiology, June 2001, p. 2344-2347, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2344-2347.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.