This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Nagendra, S.
Right arrow Articles by Doern, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nagendra, S.
Right arrow Articles by Doern, G.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, June 2001, p. 2344-2347, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2344-2347.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Sampling Variability in the Microbiological Evaluation of Expectorated Sputa and Endotracheal Aspirates

Sanjai Nagendra,1 Paul Bourbeau,2 Stephen Brecher,3 Michael Dunne,4 Mark LaRocco,5 and Gary Doern1,*

University of Iowa College of Medicine, Iowa City, Iowa1; Geisinger Medical Center, Danville, Pennsylvania2; Veterans Administration Medical Center, Boston, Massachusetts3; Henry Ford Health System, Detroit, Michigan4; and St. Lukes Episcopal Medical Center, Houston, Texas5

Received 1 November 2000/Returned for modification 2 March 2001/Accepted 20 March 2001

A five-center study was conducted with the aim of determining how reproducibly expectorated sputa and tracheal aspirates could be sampled when preparing Gram-stained smears and inoculating cultures. With both specimen types, excessive variation was noted among Gram stain results obtained from replicate smears. Less variation was noted among culture results, especially with tracheal aspirates.

* Corresponding author. Mailing address: Medical Microbiology Division, C606 GH, Department of Pathology, University of Iowa College of Medicine, Iowa City, IA 52242. Phone: (319) 356-8616. Fax: (319) 356-4916. E-mail: gary-doern{at}uiowa.edu.

Journal of Clinical Microbiology, June 2001, p. 2344-2347, Vol. 39, No. 6
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.6.2344-2347.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Munson, E., Block, T., Basile, J., Hryciuk, J. E., Schell, R. F. (2007). Mechanisms To Assess Gram Stain Interpretation Proficiency of Technologists at Satellite Laboratories. J. Clin. Microbiol. 45: 3754-3758 [Abstract] [Full Text]  
  • Woodhead, M., Blasi, F., Ewig, S., Huchon, G., Leven, M., Ortqvist, A., Schaberg, T., Torres, A., van der Heijden, G., Verheij, T. J. M. (2005). Guidelines for the management of adult lower respiratory tract infections. Eur Respir J 26: 1138-1180 [Abstract] [Full Text]  
  • Klaschik, S., Lehmann, L. E., Raadts, A., Book, M., Hoeft, A., Stuber, F. (2002). Real-Time PCR for Detection and Differentiation of Gram-Positive and Gram-Negative Bacteria. J. Clin. Microbiol. 40: 4304-4307 [Abstract] [Full Text]  
  • Carroll, K. C. (2002). Laboratory Diagnosis of Lower Respiratory Tract Infections: Controversy and Conundrums. J. Clin. Microbiol. 40: 3115-3120 [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»