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Journal of Clinical Microbiology, July 2001, p. 2397-2404, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2397-2404.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Truncated Hantavirus Nucleocapsid Proteins for Serotyping Hantaan, Seoul, and Dobrava Hantavirus Infections

Koichi Araki,1 Kumiko Yoshimatsu,2 Michiko Ogino,2 Hideki Ebihara,2 Åke Lundkvist,3 Hiroaki Kariwa,1 Ikuo Takashima,1 and Jiro Arikawa2,*

Institute for Animal Experimentation, Hokkaido University School of Medicine, Hokkaido University, Sapporo 060-8638,2 and Laboratory of Public Health, Department of Environmental Veterinary Sciences, Graduate School of Veterinary Medicine, Hokkaido University, Sapporo 060-0818,1 Japan, and Swedish Institute for Infectious Disease Control and Microbiology and Tumorbiology Center, Karolinska Institute, Stockholm, Sweden3

Received 18 September 2000/Returned for modification 2 March 2001/Accepted 23 April 2001

Truncated recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Seoul virus (SEOV), and Dobrava virus (DOBV) were expressed by a baculovirus system. The truncated rNPs, which lacked 49 (rNP50) or 154 (rNP155) N-terminal amino acids of the NPs of HTNV, SEOV, and DOBV, were able to differentiate HTNV-, SEOV-, and DOBV-specific immune sera. Recombinant NP50s retained higher reactivities than rNP155s and were proven useful for enzyme-linked immunosorbent assay (ELISA). The ELISAs based on the rNP50s of HTNV, SEOV, and DOBV successfully differentiated three groups of patient sera, previously defined by neutralization tests: 17 with HTNV infection, 12 with SEOV infection, and 20 with DOBV infection. The entire rNP of Puumala virus (PUUV) distinguished PUUV infection from the other types of hantavirus infection. Serotyping with these rNP50s can be recommended as a rapid and efficient system for hantavirus diagnosis.

* Corresponding author. Mailing address: Institute for Animal Experimentation, Hokkaido University School of Medicine, Hokkaido University, Kita-15, Nishi-7, Kita ku, Sapporo 060-8638, Japan. Phone: 81-11-706-6905. Fax: 81-11-706-7879. E-mail: j_arika{at}med.hokudai.ac.jp.

Journal of Clinical Microbiology, July 2001, p. 2397-2404, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2397-2404.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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