JCM Figure table search 04
Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barlow, R. E. L.
Right arrow Articles by Hawkey, P. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barlow, R. E. L.
Right arrow Articles by Hawkey, P. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2001, p. 2453-2457, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2453-2457.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Variable Number Tandem Repeat and IS6110-Restriction Fragment Length Polymorphism Analyses for Discrimination of High- and Low-Copy-Number IS6110 Mycobacterium tuberculosis Isolates

Rachael E. L. Barlow,1 Deborah M. Gascoyne-Binzi,1,2,* Stephen H. Gillespie,3 Anne Dickens,3 Shabnam Qamer,1 and Peter M. Hawkey1,2

The Division of Microbiology, School of Biochemistry & Molecular Biology, University of Leeds, Leeds LS2 9JT,1 Department of Microbiology, The General Infirmary, Leeds LS1 3EX,2 and Department of Medical Microbiology, Royal Free & University College Medical School, University College London, Royal Free Campus, London NW3 2PE,3 United Kingdom

Received 16 October 2000/Returned for modification 24 January 2001/Accepted 29 April 2001

The present study was designed to evaluate the use of variable number tandem repeat (VNTR) and IS6110-restriction fragment length polymorphism (RFLP) analyses in combination as a two-step strategy for discrimination (as measured by the Hunter-Gaston Discrimination Index [HGDI]) of both high- and low-copy-number IS6110 Mycobacterium tuberculosis isolates compared to IS6110-RFLP alone with an unselected collection of isolates. Individually, IS6110-RFLP fingerprinting produced six clusters that accounted for 69% of the low-copy-number IS6110 isolates (five clusters) and 5% of the high-copy-number IS6110 isolates (one cluster). A total of 39% of all the isolates were clustered (HGDI = 0.97). VNTR analysis generated a total of 35 different VNTR allele profile sets from 93 isolates (HGDI = 0.938). Combining IS6110-RFLP analysis with VNTR analysis reduced the overall percentage of clustered isolates to 29% (HGDI = 0.988) and discriminated a further 27% of low-copy-number isolates that would have been clustered by IS6110-RFLP alone. The use of VNTR analysis as an initial typing strategy facilitates further analysis by IS6110-RFLP, and more importantly, VNTR analysis subdivides some IS6110-RFLP-defined clusters containing low- and single-copy IS6110 isolates.

* Corresponding author. Mailing address: The Division of Microbiology, Department of Microbiology, The General Infirmary, Great George Street, Leeds LS1 3EX, United Kingdom. Phone: (44)-113-2335592. Fax: (44)-113-2335638. E-mail: deborahg{at}pathology.leeds.ac.uk.

Journal of Clinical Microbiology, July 2001, p. 2453-2457, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2453-2457.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:



Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
Clin. Vaccine Immunol. ALL ASM JOURNALS

Copyright © 2001 by the American Society for Microbiology. All rights reserved.