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Journal of Clinical Microbiology, July 2001, p. 2594-2597, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2594-2597.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of RTX Toxin Gene in Vibrio cholerae by PCR

K. H. Chow,1 T. K. Ng,2 K. Y. Yuen,1 and W. C. Yam1,*

Department of Microbiology, The University of Hong Kong,1 and Department of Pathology, Princess Margaret Hospital,2 Hong Kong SAR, China

Received 30 January 2001/Returned for modification 6 March 2001/Accepted 24 April 2001

A PCR that amplifies a recently discovered Vibrio cholerae RTX (repeat in toxin) toxin gene was developed. Among 166 clinical and environmental isolates of V. cholerae causing epidemics and sporadic cases of cholera in various parts of the world, all were found to be toxigenic by both PCR and HEp-2 cell cytotoxicity assay. Standard strains of the classical biotype containing a deletion within the gene cluster exhibited negative results by both assays. This is the first rapid genotyping method for differentiation of V. cholerae O1 classical biotype strains from El Tor biotype strains as well as strains of other non-O1 serogroups including serogroup O139. The PCR assay that was developed also specifically detects RTX toxin genes in V. cholerae, as clinical isolates of Vibrio parahaemolyticus, diarrheagenic Escherichia coli, Aeromonas species, and Plesiomonas species were all negative by the RTX toxin-specific PCR as well as the HEp-2 cytotoxicity assay. These findings highlight the characteristics of the RTX toxins in V. cholerae. Their role in the pathogenicity of the bacterium requires further investigation.

* Corresponding author. Mailing address: Department of Microbiology, Queen Mary Hospital, The University of Hong Kong, Pokfulam, Hong Kong SAR, China. Phone: (852) 2855 4892. Fax: (852) 2855 1241. E-mail: wcyam{at}hkucc.hku.hk.

Journal of Clinical Microbiology, July 2001, p. 2594-2597, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2594-2597.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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