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Journal of Clinical Microbiology, July 2001, p. 2642-2645, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2642-2645.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Mycolic Acid Index Susceptibility Method for Mycobacterium tuberculosis

José M. Viader-Salvadó,1,* Elvira Garza-González,1 Ramón Valdez-Leal,2 M. de los Angeles del Bosque-Moncayo,2 Rolando Tijerina-Menchaca,1 and Martha Guerrero-Olazarán1

Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Monterrey,1 and Laboratorio Estatal de Salud Pública de los Servicios de Salud de Nuevo León, Guadalupe,2 Nuevo León, México

Received 21 December 2000/Returned for modification 6 February 2001/Accepted 24 April 2001

A rapid drug susceptibility test to measure the susceptibility of Mycobacterium tuberculosis to isoniazid (INH) and rifampin (RIF) using clinical isolates and a newly defined mycolic acid index (MAI) was evaluated. A total of 200 clinical isolates of M. tuberculosis were tested for susceptibility or resistance to INH and RIF by the MAI susceptibility and indirect-proportion methods. Overall, there was agreement between the two methods for 398 (99.5%) of the 400 total tests. Specifically, the sensitivity of the MAI susceptibility method for INH and RIF was 97.6 and 100%, respectively. The specificity and positive predictive value were 100% for both drugs, and the negative predictive value for INH and RIF was 98.3 and 100%, respectively. In conclusion, the MAI susceptibility method described here can be used for rapid drug susceptibility testing of M. tuberculosis clinical isolates within 5 days after clinical isolates are incubated in the presence or absence of an antituberculosis drug.

* Corresponding author. Mailing address: Departamento de Microbiologia, Facultad de Medicina, U.A.N.L., Av. Madero y Dr. E. Aguirre Pequeno, Col. Mitras Centro, 64460 Monterrey, N.L., Mexico. Phone and fax: (528) 329-4050, ext. 2587. E-mail: jviader{at}ccr.dsi.uanl.mx.

Journal of Clinical Microbiology, July 2001, p. 2642-2645, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2642-2645.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.