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Journal of Clinical Microbiology, July 2001, p. 2655-2659, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2655-2659.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of a Reverse Dot Blot Procedure To Identify the Presence of Multiple Serovars in Chlamydia trachomatis Urogenital Infection

Diane R. Stothard*

Department of Medicine, Division of Infectious Diseases, Indiana University School of Medicine, Indianapolis, Indiana 46202

Received 18 September 2000/Returned for modification 22 January 2001/Accepted 23 April 2001

Epidemiologic research requires identification of Chlamydia trachomatis serovars and detection of mixed infection. Antibody-based serotyping is unworkable when specimens are urine or vaginal swabs. We developed a reverse dot blot (RDB) to screen for multiple serotypes in these specimens. RDB yielded the predicted results on all artificially mixed samples and on seven of eight clinically mixed samples.

* Mailing address: Department of Medicine, Division of Infectious Diseases, Indiana University School of Medicine, 435 Emerson Hall, 545 Barnhill Dr., Indianapolis, IN 46202. Phone: (317) 274-7673. Fax: (317) 275-1587. E-mail: dstothar{at}iupui.edu.

Journal of Clinical Microbiology, July 2001, p. 2655-2659, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2655-2659.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Jalal, H., Stephen, H., Alexander, S., Carne, C., Sonnex, C. (2007). Development of Real-Time PCR Assays for Genotyping of Chlamydia trachomatis. J. Clin. Microbiol. 45: 2649-2653 [Abstract] [Full Text]  
  • Xiong, L., Kong, F., Zhou, H., Gilbert, G. L. (2006). Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis. J. Clin. Microbiol. 44: 1413-1418 [Abstract] [Full Text]  


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