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Journal of Clinical Microbiology, July 2001, p. 2675-2676, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2675-2676.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Use of Real-Time Quantitative PCR To Detect Chlamydophila felis Infection

Chris Helps,* Nicky Reeves, Séverine Tasker, and Dave Harbour

Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford, Bristol, BS40 5DU, United Kingdom

Received 11 December 2000/Returned for modification 8 April 2001/Accepted 29 April 2001

A real-time PCR assay was developed to detect and quantify Chlamydophila felis infection of cats. The assay uses a molecular beacon to specifically identify the major outer membrane protein gene, is highly reproducible, and is able to detect fewer than 10 genomic copies.

* Corresponding author. Mailing address: Division of Molecular and Cellular Biology, Department of Clinical Veterinary Science, University of Bristol, Langford House, Langford, Bristol, BS40 5DU, United Kingdom. Phone: 44 117 928 9242. Fax: 44 117 928 9505. E-mail: c.r.helps{at}Bristol.ac.uk.

Journal of Clinical Microbiology, July 2001, p. 2675-2676, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2675-2676.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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