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Journal of Clinical Microbiology, July 2001, p. 2683-2686, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2683-2686.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Comparison of Restriction Fragment Length Polymorphism, Microsatellite Length Polymorphism, and Random Amplification of Polymorphic DNA Analyses for Fingerprinting Aspergillus fumigatus Isolates

Emmanuelle Bart-Delabesse,1 Jacqueline Sarfati,2 Jean-Paul Debeaupuis,2 Willem van Leeuwen,3 Alex van Belkum,3 Stephane Bretagne,1 and Jean-Paul Latge2,*

Laboratoire de Parasitologie-Mycologie, Hôpital Henri Mondor, Créteil,1 and Unité des Aspergillus, Institut Pasteur, Paris,2 France, and Department of Medical Microbiology and Infectious Diseases, Erasmus Medical Center, Rotterdam, The Netherlands3

Received 21 December 2000/Returned for modification 18 March 2001/Accepted 24 April 2001

Aspergillus fumigatus fingerprints generated by random amplification of polymorphic DNA (RAPD), restriction fragment length polymorphism (RFLP) upon hybridization with repeated DNA sequences, and PCR detection of microsatellite length polymorphism (MLP) were compared among 67 isolates. In contrast to RAPD, RFLP and MLP gave discriminating and significantly concordant genotyping results.

* Corresponding author. Mailing address: Unité des Aspergillus, Institut Pasteur, 25, rue du docteur Roux, 75724 Paris cedex, France. Phone: 33 1 40 61 35 18. Fax: 33 1 40 61 34 19. E-mail: jplatge{at}pasteur.fr.

Journal of Clinical Microbiology, July 2001, p. 2683-2686, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2683-2686.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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