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Journal of Clinical Microbiology, July 2001, p. 2708-2712, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2708-2712.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Novel Fluorescent Broth Microdilution Method for Fluconazole Susceptibility Testing of Candida albicans

Robert S. Liao,1 Robert P. Rennie,1,2,* and James A. Talbot1

Department of Medical Microbiology and Immunology,1 University of Alberta, and National Centre for Mycology, University of Alberta Hospital, Walter C. Mackenzie Health Sciences Centre,2 Edmonton, Alberta T6G 2J2, Canada

Received 13 October 2000/Returned for modification 5 March 2001/Accepted 19 April 2001

A comparative evaluation of the reference National Committee for Clinical Laboratory Standards (NCCLS) broth microdilution method with a novel fluorescent carboxyfluorescein diacetate (CFDA)-modified microdilution method for the susceptibility testing of fluconazole was conducted with 68 Candida strains, including 53 Candida albicans, 5 Candida tropicalis, 5 Candida glabrata, and 5 Candida parapsilosis strains. We found trailing endpoints and discordant fluconazole MICs of <8 µg/ml at 24 h and of >= 64 µg/ml at 48 h for 12 of the C. albicans strains. These strains satisfy the definition of the low-high MIC phenotype. All 12 low-high phenotype strains were correctly shown to be susceptible at 48 h with the CFDA-modified microdilution method. For the 41 non-low-high phenotype C. albicans strains, the CFDA-modified microdilution method yielded 97.6% (40 of 41 strains) agreement within ±1 dilution at 24 h compared with the reference method and 92.7% (38 of 41 strains) agreement within ±1 dilution at 48 h compared with the reference method. The five strains each from C. tropicalis, C. glabrata, and C. parapsilosis that were tested showed 100% agreement within ±2 dilutions for the two methods being evaluated.

* Corresponding author. Present address: Department of Microbiology and Public Health, 2B3.08 Walter Mackenzie Centre, University of Alberta Hospital, 8440-112 St., Edmonton, Alberta T6G 2J2, Canada. Phone: (780) 407-7242. Fax: (780) 407-3864. E-mail: rpr{at}bugs.uah.ualberta.ca.

Journal of Clinical Microbiology, July 2001, p. 2708-2712, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2708-2712.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Peter, J., Armstrong, D., Lyman, C. A., Walsh, T. J. (2005). Use of Fluorescent Probes To Determine MICs of Amphotericin B and Caspofungin against Candida spp. and Aspergillus spp.. J. Clin. Microbiol. 43: 3788-3792 [Abstract] [Full Text]  
  • Liao, R. S., Rennie, R. P., Talbot, J. A. (2002). Comparative Evaluation of a New Fluorescent Carboxyfluorescein Diacetate-Modified Microdilution Method for Antifungal Susceptibility Testing of Candida albicans Isolates. Antimicrob. Agents Chemother. 46: 3236-3242 [Abstract] [Full Text]  
  • Balajee, S. A., Marr, K. A. (2002). Conidial Viability Assay for Rapid Susceptibility Testing of Aspergillus Species. J. Clin. Microbiol. 40: 2741-2745 [Abstract] [Full Text]  


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