This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hawser, S. P.
Right arrow Articles by Ghannoum, M. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hawser, S. P.
Right arrow Articles by Ghannoum, M. A.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, July 2001, p. 2738-2741, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2738-2741.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Utility of 2,3-Bis(2-Methoxy-4-Nitro-5-Sulfophenyl)-5-[(Phenyl-Amino)Carbonyl]-2H-Tetrazolium Hydroxide (XTT) and Minimum Effective Concentration Assays in the Determination of Antifungal Susceptibility of Aspergillus fumigatus to the Lipopeptide Class of Compounds

S. P. Hawser,1 C. Jessup,2 J. Vitullo,2 and M. A. Ghannoum2,3,*

Aventis, F-93235 Romainville, France,1 and Mycology Reference Laboratory, Center for Medical Mycology, University Hospitals of Cleveland,3 and Department of Dermatology, Case Western Reserve University,2 Cleveland, Ohio 44106-5028

Received 1 September 2000/Returned for modification 9 February 2001/Accepted 19 April 2001

The susceptibility of Aspergillus fumigatus to mulundocandin, an echinocandin-like compound, and other antifungal agents was assessed by the National Committee for Clinical Laboratory Standards (NCCLS) M38-P method, a 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl-amino)carbonyl]-2H-tetrazolium hydroxide (XTT)-based colorimetric assay, and determination of morphologic alterations by microscopy. In contrast to the NCCLS M38-P method, which does not predict the activity in vivo, the XTT-based assay showed that A. fumigatus is susceptible to mulundocandin. Thus, the XTT-based assay might be useful for determination of the susceptibilities of molds to echinocandins. Further evaluation is warranted.

* Corresponding author. Mailing address: Mycology Reference Laboratory, Center for Medical Mycology, University Hospitals of Cleveland, 11100 Euclid Ave., LKS 5028, Cleveland, OH 44106-5028. Phone: (216) 844-8580. Fax: (216) 844-1076. E-mail: mag3{at}po.cwru.edu.

Journal of Clinical Microbiology, July 2001, p. 2738-2741, Vol. 39, No. 7
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.7.2738-2741.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Shehata, A. S., Mukherjee, P. K., Ghannoum, M. A. (2008). Comparison between the Standardized Clinical and Laboratory Standards Institute M38-A2 Method and a 2,3-Bis(2-Methoxy-4-Nitro-5-[(Sulphenylamino)Carbonyl]-2H-Tetrazolium Hydroxide- Based Method for Testing Antifungal Susceptibility of Dermatophytes. J. Clin. Microbiol. 46: 3668-3671 [Abstract] [Full Text]  
  • Mowat, E., Butcher, J., Lang, S., Williams, C., Ramage, G. (2007). Development of a simple model for studying the effects of antifungal agents on multicellular communities of Aspergillus fumigatus. J Med Microbiol 56: 1205-1212 [Abstract] [Full Text]  
  • Ahmed, A. O. A., van de Sande, W. W. J., van Vianen, W., van Belkum, A., Fahal, A. H., Verbrugh, H. A., Bakker-Woudenberg, I. A. J. M. (2004). In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay. Antimicrob. Agents Chemother. 48: 2742-2746 [Abstract] [Full Text]  
  • Tunney, M. M., Ramage, G., Field, T. R., Moriarty, T. F., Storey, D. G. (2004). Rapid Colorimetric Assay for Antimicrobial Susceptibility Testing of Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 48: 1879-1881 [Abstract] [Full Text]  
  • Johnson, M. D., MacDougall, C., Ostrosky-Zeichner, L., Perfect, J. R., Rex, J. H. (2004). Combination Antifungal Therapy. Antimicrob. Agents Chemother. 48: 693-715 [Full Text]  
  • Arikan, S., Paetznick, V., Rex, J. H. (2002). Comparative Evaluation of Disk Diffusion with Microdilution Assay in Susceptibility Testing of Caspofungin against Aspergillus and Fusarium Isolates. Antimicrob. Agents Chemother. 46: 3084-3087 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»