This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Brunello, F.
Right arrow Articles by Fontana, R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Brunello, F.
Right arrow Articles by Fontana, R.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, August 2001, p. 2799-2806, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2799-2806.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of 54 Mycobacterial Species by PCR-Restriction Fragment Length Polymorphism Analysis of the hsp65 Gene

Francesca Brunello,1 Marco Ligozzi,1 Emanuela Cristelli,1 Stefano Bonora,2 Enrico Tortoli,3 and Roberta Fontana1,*

Dipartimento di Patologia, Sezione di Microbiologia, Università di Verona and Servizio di Microbiologia dell'Azienda Ospedaliera di Verona, Verona,1 Clinica delle Malattie Infettive, Università di Torino, Turin,2 and Servizio di Microbiologia, Ospedale Careggi Firenze e Centro di Riferimento Nazionale per i Micobatteri, Florence,3 Italy

Received 19 March 2001/Returned for modification 29 April 2001/Accepted 28 May 2001

A total of 121 reference and clinical strains of both slowly and rapidly growing mycobacteria belonging to 54 species were studied for restriction fragment length polymorphism of a PCR-amplified 439-bp segment of the gene encoding the 65-kDa heat shock protein. Restriction digests were separated by 10% polyacrylamide gel electrophoresis (PAGE). By including a size standard in each sample, the restriction fragment profile was calculated using a computer-aided comparison program. An algorithm describing these 54 species (including 22 species not previously described) is proposed. We found that this assay based on 10% PAGE provided a more precise estimate than that based on agarose gel electrophoresis of the real size of restriction fragments as deduced from the sequence analysis and allowed identification of mycobacteria whose PCR-restriction fragment length polymorphism analysis patterns were unequivocally identified by fragments shorter than 60 bp.

* Corresponding author. Mailing address: Università di Verona, Dipartimento di Patologia, Sezione di Microbiologia; Strada le Grazie 8, 37100 Verona, Italy. Phone: 0039-45-8028191. Fax: 0039-45-584606. E-mail: roberta.fontana{at}univr.it.

Journal of Clinical Microbiology, August 2001, p. 2799-2806, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2799-2806.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Choi, P., Qin, X., Chen, E. Y., Inglis, A. F. Jr, Ou, H. C., Perkins, J. A., Sie, K. C. Y., Patterson, K., Berry, S., Manning, S. C. (2009). Polymerase Chain Reaction for Pathogen Identification in Persistent Pediatric Cervical Lymphadenitis. Arch Otolaryngol Head Neck Surg 135: 243-248 [Abstract] [Full Text]  
  • Wu, T.-L., Chia, J.-H., Kuo, A.-J., Su, L.-H., Wu, T.-S., Lai, H.-C. (2008). Rapid Identification of Mycobacteria from Smear-Positive Sputum Samples by Nested PCR-Restriction Fragment Length Polymorphism Analysis. J. Clin. Microbiol. 46: 3591-3594 [Abstract] [Full Text]  
  • Ventura, M., Canchaya, C., Tauch, A., Chandra, G., Fitzgerald, G. F., Chater, K. F., van Sinderen, D. (2007). Genomics of Actinobacteria: Tracing the Evolutionary History of an Ancient Phylum. Microbiol. Mol. Biol. Rev. 71: 495-548 [Abstract] [Full Text]  
  • Pignone, M., Greth, K. M., Cooper, J., Emerson, D., Tang, J. (2006). Identification of Mycobacteria by Matrix-Assisted Laser Desorption Ionization-Time-of-Flight Mass Spectrometry.. J. Clin. Microbiol. 44: 1963-1970 [Abstract] [Full Text]  
  • Rodriguez-Nava, V., Couble, A., Devulder, G., Flandrois, J.-P., Boiron, P., Laurent, F. (2006). Use of PCR-Restriction Enzyme Pattern Analysis and Sequencing Database for hsp65 Gene-Based Identification of Nocardia Species. J. Clin. Microbiol. 44: 536-546 [Abstract] [Full Text]  
  • McNabb, A., Adie, K., Rodrigues, M., Black, W. A., Isaac-Renton, J. (2006). Direct Identification of Mycobacteria in Primary Liquid Detection Media by Partial Sequencing of the 65-Kilodalton Heat Shock Protein Gene. J. Clin. Microbiol. 44: 60-66 [Abstract] [Full Text]  
  • Khan, I. U. H., Selvaraju, S. B., Yadav, J. S. (2005). Method for Rapid Identification and Differentiation of the Species of the Mycobacterium chelonae Complex Based on 16S-23S rRNA Gene Internal Transcribed Spacer PCR-Restriction Analysis. J. Clin. Microbiol. 43: 4466-4472 [Abstract] [Full Text]  
  • Konig, B., Tammer, I., Sollich, V., Konig, W. (2005). Intra- and Interpatient Variability of the hsp65 and 16S-23S Intergenic Gene Region in Mycobacterium abscessus Strains from Patients with Cystic Fibrosis. J. Clin. Microbiol. 43: 3500-3503 [Abstract] [Full Text]  
  • Rhodes, M. W., Kator, H., McNabb, A., Deshayes, C., Reyrat, J.-M., Brown-Elliott, B. A., Wallace, R. Jr, Trott, K. A., Parker, J. M., Lifland, B., Osterhout, G., Kaattari, I., Reece, K., Vogelbein, W., Ottinger, C. A. (2005). Mycobacterium pseudoshottsii sp. nov., a slowly growing chromogenic species isolated from Chesapeake Bay striped bass (Morone saxatilis). Int. J. Syst. Evol. Microbiol. 55: 1139-1147 [Abstract] [Full Text]  
  • Devulder, G., de Montclos, M. P., Flandrois, J. P. (2005). A multigene approach to phylogenetic analysis using the genus Mycobacterium as a model. Int. J. Syst. Evol. Microbiol. 55: 293-302 [Abstract] [Full Text]  
  • Adekambi, T., Drancourt, M. (2004). Dissection of phylogenetic relationships among 19 rapidly growing Mycobacterium species by 16S rRNA, hsp65, sodA, recA and rpoB gene sequencing. Int. J. Syst. Evol. Microbiol. 54: 2095-2105 [Abstract] [Full Text]  
  • Ho, H.-T., Chang, P.-L., Hung, C.-C., Chang, H.-T. (2004). Capillary Electrophoretic Restriction Fragment Length Polymorphism Patterns for the Mycobacterial hsp65 Gene. J. Clin. Microbiol. 42: 3525-3531 [Abstract] [Full Text]  
  • McNabb, A., Eisler, D., Adie, K., Amos, M., Rodrigues, M., Stephens, G., Black, W. A., Isaac-Renton, J. (2004). Assessment of Partial Sequencing of the 65-Kilodalton Heat Shock Protein Gene (hsp65) for Routine Identification of Mycobacterium Species Isolated from Clinical Sources. J. Clin. Microbiol. 42: 3000-3011 [Abstract] [Full Text]  
  • Wang, S. X., Sng, L. H., Leong, H. N., Tan, B. H. (2004). Direct Identification of Mycobacterium haemophilum in Skin Lesions of Immunocompromised Patients by PCR-Restriction Endonuclease Analysis. J. Clin. Microbiol. 42: 3336-3338 [Abstract] [Full Text]  
  • Turenne, C. Y., Thibert, L., Williams, K., Burdz, T. V., Cook, V. J., Wolfe, J. N., Cockcroft, D. W., Kabani, A. (2004). Mycobacterium saskatchewanense sp. nov., a novel slowly growing scotochromogenic species from human clinical isolates related to Mycobacterium interjectum and Accuprobe-positive for Mycobacterium avium complex. Int. J. Syst. Evol. Microbiol. 54: 659-667 [Abstract] [Full Text]  
  • Turenne, C. Y., Suchak, A. A., Wolfe, J. N., Kabani, A., Nicolle, L. E. (2003). Soft Tissue Infection Caused by a Novel Pigmented, Rapidly Growing Mycobacterium Species. J. Clin. Microbiol. 41: 2779-2782 [Abstract] [Full Text]  
  • Hall, L., Doerr, K. A., Wohlfiel, S. L., Roberts, G. D. (2003). Evaluation of the MicroSeq System for Identification of Mycobacteria by 16S Ribosomal DNA Sequencing and Its Integration into a Routine Clinical Mycobacteriology Laboratory. J. Clin. Microbiol. 41: 1447-1453 [Abstract] [Full Text]  
  • Huard, R. C., de Oliveira Lazzarini, L. C., Butler, W. R., van Soolingen, D., Ho, J. L. (2003). PCR-Based Method To Differentiate the Subspecies of the Mycobacterium tuberculosis Complex on the Basis of Genomic Deletions. J. Clin. Microbiol. 41: 1637-1650 [Abstract] [Full Text]  
  • Itoh, S., Kazumi, Y., Abe, C., Takahashi, M. (2003). Heterogeneity of RNA Polymerase Gene (rpoB) Sequences of Mycobacterium gordonae Clinical Isolates Identified with a DNA Probe Kit and by Conventional Methods. J. Clin. Microbiol. 41: 1656-1663 [Abstract] [Full Text]  
  • Taillard, C., Greub, G., Weber, R., Pfyffer, G. E., Bodmer, T., Zimmerli, S., Frei, R., Bassetti, S., Rohner, P., Piffaretti, J.-C., Bernasconi, E., Bille, J., Telenti, A., Prod'hom, G. (2003). Clinical Implications of Mycobacterium kansasii Species Heterogeneity: Swiss National Survey. J. Clin. Microbiol. 41: 1240-1244 [Abstract] [Full Text]  
  • Ucko, M., Colorni, A., Kvitt, H., Diamant, A., Zlotkin, A., Knibb, W. R. (2002). Strain Variation in Mycobacteriummarinum Fish Isolates. Appl. Environ. Microbiol. 68: 5281-5287 [Abstract] [Full Text]  
  • da Silva Rocha, A., Werneck Barreto, A. M., Dias Campos, C. E., Villas-Boas da Silva, M., Fonseca, L., Saad, M. H., Degrave, W. M., Suffys, P. N. (2002). Novel Allelic Variants of Mycobacteria Isolated in Brazil as Determined by PCR-Restriction Enzyme Analysis of hsp65. J. Clin. Microbiol. 40: 4191-4196 [Abstract] [Full Text]  
  • Francois, A., Mathis, H., Godefroy, D., Piveteau, P., Fayolle, F., Monot, F. (2002). Biodegradation of Methyl tert-Butyl Ether and Other Fuel Oxygenates by a New Strain, Mycobacterium austroafricanum IFP 2012. Appl. Environ. Microbiol. 68: 2754-2762 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»