Malaria Rapid Diagnostic Devices: Performance Characteristics of the ParaSight F Device Determined in a Multisite Field Study

doi:10.1128/JCM.39.8.2884-2890.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Forney, J. R.
	Articles by Gasser, R. A.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Forney, J. R.
	Articles by Gasser, R. A., Jr.

Journal of Clinical Microbiology, August 2001, p. 2884-2890, Vol. 39, No. 8
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.8.2884-2890.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Malaria Rapid Diagnostic Devices: Performance Characteristics of the ParaSight F Device Determined in a Multisite Field Study

J. Russ Forney,¹^,^* Alan J. Magill,² Chansuda Wongsrichanalai,³ Jeeraphat Sirichaisinthop,⁴ Christian T. Bautista,² D. Gray Heppner,¹ R. Scott Miller,⁵ Christian F. Ockenhouse,¹ Alex Gubanov,⁶ Robyn Shafer,⁶ Caroline Cady DeWitt,⁷ Higinio A. Quino-Ascurra,⁸ Kent E. Kester,¹ Kevin C. Kain,⁶ Douglas S. Walsh,³ W. Ripley Ballou,¹ and Robert A. Gasser Jr.¹

Walter Reed Army Institute of Research¹ and Walter Reed Army Medical Center,⁵ Washington, D.C.; Naval Medical Research Center Detachment, Lima,² and Hospital de Apoyo, Iquitos,⁸ Peru; Armed Forces Research Institute of Medical Sciences, Bangkok,³ and Vector Borne Disease Control Office #1, Phrabuddhabat, Saraburi,⁴ Thailand; The Toronto General Hospital and University of Toronto, Toronto, Canada⁶; and Wilford Hall Medical Center, Lackland Air Force Base, Texas⁷

Received 16 February 2001/Returned for modification 3 April 2001/Accepted 30 May 2001

Microscopic detection of parasites has been the reference standard for malaria diagnosis for decades. However, difficultyin maintaining required technical skills and infrastructure hasspurred the development of several nonmicroscopic malaria rapiddiagnostic devices based on the detection of malaria parasiteantigen in whole blood. The ParaSight F test is one such device.It detects the presence of Plasmodium falciparum-specific histidine-richprotein 2 by using an antigen-capture immunochromatographic stripformat. The present study was conducted at outpatient malariaclinics in Iquitos, Peru, and Maesod, Thailand. Duplicate, blinded,expert microscopy was employed as the reference standard for evaluatingdevice performance. Of 2,988 eligible patients, microscopy showedthat 547 (18%) had P. falciparum, 658 (22%) had P. vivax, 2 (0.07%)had P. malariae, and 1,750 (59%) were negative for Plasmodium.Mixed infections (P. falciparum and P. vivax) were identifiedin 31 patients (1%). The overall sensitivity of ParaSight F forP. falciparum was 95%. When stratified by magnitude of parasitemia(no. of asexual parasites per microliter of whole blood), sensitivitieswere 83% (>0 to 500 parasites/µl), 87% (501 to 1,000/µl), 98%(1,001 to 5,000/µl), and 98% (>5,000/µl). Device specificity was86%.

^* Corresponding author. Mailing address: MADN-CHEM, United States Military Academy, Official Mail & Distribution Center, 646Swift Rd., West Point, NY 10996-1905. Phone: (845) 938-8298. Fax:(845) 938-2235. E-mail: mj9173{at}usma.edu.

This article has been cited by other articles:

Wongsrichanalai, C., Barcus, M. J., Muth, S., Sutamihardja, A., Wernsdorfer, W. H. (2007). A Review of Malaria Diagnostic Tools: Microscopy and Rapid Diagnostic Test (RDT). Am J Trop Med Hyg 77: 119-127 [Abstract] [Full Text]
Lee, N., Baker, J., Andrews, K. T., Gatton, M. L., Bell, D., Cheng, Q., McCarthy, J. (2006). Effect of Sequence Variation in Plasmodium falciparum Histidine- Rich Protein 2 on Binding of Specific Monoclonal Antibodies: Implications for Rapid Diagnostic Tests for Malaria.. J. Clin. Microbiol. 44: 2773-2778 [Abstract] [Full Text]
MILLER, R. S., WONGSRICHANALAI, C., BUATHONG, N., McDANIEL, P., WALSH, D. S., KNIRSCH, C., OHRT, C. (2006). EFFECTIVE TREATMENT OF UNCOMPLICATED PLASMODIUM FALCIPARUM MALARIA WITH AZITHROMYCIN-QUININE COMBINATIONS: A RANDOMIZED, DOSE-RANGING STUDY.. Am J Trop Med Hyg 74: 401-406 [Abstract] [Full Text]
O'MEARA, W. P., MCKENZIE, F. E., MAGILL, A. J., FORNEY, J. R., PERMPANICH, B., LUCAS, C., GASSER, R. A. JR., WONGSRICHANALAI, C. (2005). SOURCES OF VARIABILITY IN DETERMINING MALARIA PARASITE DENSITY BY MICROSCOPY. Am J Trop Med Hyg 73: 593-598 [Abstract] [Full Text]
SCHOLL, P. F., KONGKASURIYACHAI, D., DEMIREV, P. A., FELDMAN, A. B., LIN, J. S., SULLIVAN, D. J. JR., KUMAR, N. (2004). RAPID DETECTION OF MALARIA INFECTION IN VIVO BY LASER DESORPTION MASS SPECTROMETRY. Am J Trop Med Hyg 71: 546-551 [Abstract] [Full Text]
McKENZIE, F. E., SIRICHAISINTHOP, J., MILLER, R. S., GASSER, R. A. JR., WONGSRICHANALAI, C. (2003). DEPENDENCE OF MALARIA DETECTION AND SPECIES DIAGNOSIS BY MICROSCOPY ON PARASITE DENSITY. Am J Trop Med Hyg 69: 372-376 [Abstract] [Full Text]
Forney, J. R., Wongsrichanalai, C., Magill, A. J., Craig, L. G., Sirichaisinthop, J., Bautista, C. T., Miller, R. S., Ockenhouse, C. F., Kester, K. E., Aronson, N. E., Andersen, E. M., Quino-Ascurra, H. A., Vidal, C., Moran, K. A., Murray, C. K., DeWitt, C. C., Heppner, D. G., Kain, K. C., Ballou, W. R., Gasser, R. A. Jr. (2003). Devices for Rapid Diagnosis of Malaria: Evaluation of Prototype Assays That Detect Plasmodium falciparum Histidine-Rich Protein 2 and a Plasmodium vivax-Specific Antigen. J. Clin. Microbiol. 41: 2358-2366 [Abstract] [Full Text]

Antimicrob. Agents Chemother.	Clin. Microbiol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS