Burkholderia cepacia Complex Infection in Italian Patients with Cystic Fibrosis: Prevalence, Epidemiology, and Genomovar Status

doi:10.1128/JCM.39.8.2891-2896.2001

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Journal of Clinical Microbiology, August 2001, p. 2891-2896, Vol. 39, No. 8
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.8.2891-2896.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Burkholderia cepacia Complex Infection in Italian Patients with Cystic Fibrosis: Prevalence, Epidemiology, and Genomovar Status

Antonella Agodi,¹ Eshwar Mahenthiralingam,² Martina Barchitta,¹ Viviana Gianninò,³ Agata Sciacca,⁴ and Stefania Stefani³^,^*

Department of Biomedical Sciences,¹ Department of Microbiological and Gynecological Sciences,³ and Microbiological Laboratory, Policlinico,⁴ University of Catania, Catania, Italy, and School of Biosciences, Cardiff University, Cardiff, Wales, United Kingdom²

Received 17 January 2001/Returned for modification 14 March 2001/Accepted 7 May 2001

The prevalence, epidemiology, and genomovar status of Burkholderia cepacia complex strains recovered from Italian cystic fibrosis(CF) patients were investigated using genetic typing and speciesidentification methods. Four CF treatment centers were examined:two in Sicily, one in central Italy, and one in northern Italy.B. cepacia complex bacteria were isolated from 59 out of 683 CFpatients attending these centers (8.6%). For the two geographicallyrelated treatment centers in Sicily, there was a high incidenceof infection caused by a single epidemic clone possessing thecblA gene and belonging to B. cepacia genomovar III, recA groupIII-A, closely related to the major North America-United Kingdomclone, ET12; instability of the cblA sequence was also demonstratedfor clonal isolates. In summary, of all the strains of B. cepaciaencountered in the Italian CF population, the genomovar III, recAgroup III-A strains were the most prevalent and transmissible.However, patient-to-patient spread was also observed with severalother genomovars, including strains of novel taxonomic statuswithin the B. cepacia complex. A combination of genetic identificationand molecular typing analysis is recommended to fully define specificrisks posed by the genomovar status of strains within the B. cepaciacomplex.

^* Corresponding author. Mailing address: Section of Microbiology of the Department of Microbiological and Gynecological Sciences,University of Catania, Via Androne 81, 95124 Catania, Italy. Phone:39 (095) 311352. Fax: 39 (095) 325032. E-mail: stefanis{at}mbox.unict.it.

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