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Journal of Clinical Microbiology, August 2001, p. 2916-2923, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2916-2923.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Identification of Enterococcus, Streptococcus, and Staphylococcus by Multivariate Analysis of Proton Magnetic Resonance Spectroscopic Data from Plate Cultures

Roger Bourne,1 Uwe Himmelreich,1 Ansuiya Sharma,2 Carolyn Mountford,1 and Tania Sorrell1,3,*

Institute for Magnetic Resonance Research and Department of Magnetic Resonance in Medicine, University of Sydney, St Leonards 2065,1 and Centre for Infectious Diseases and Microbiology (CIDM) Laboratory Services, Institute for Clinical Pathology and Medical Research,2 and CIDM,3 University of Sydney at Westmead Hospital, Sydney 2145, Australia

Received 14 February 2001/Returned for modification 22 April 2001/Accepted 26 May 2001

A new fingerprinting technique with the potential for rapid identification of bacteria was developed by combining proton magnetic resonance spectroscopy (1H MRS) with multivariate statistical analysis. This resulted in an objective identification strategy for common clinical isolates belonging to the bacterial species Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus faecalis, Streptococcus pneumoniae, Streptococcus pyogenes, Streptococcus agalactiae, and the Streptococcus milleri group. Duplicate cultures of 104 different isolates were examined one or more times using 1H MRS. A total of 312 cultures were examined. An optimized classifier was developed using a bootstrapping process and a seven-group linear discriminant analysis to provide objective classification of the spectra. Identification of isolates was based on consistent high-probability classification of spectra from duplicate cultures and achieved 92% agreement with conventional methods of identification. Fewer than 1% of isolates were identified incorrectly. Identification of the remaining 7% of isolates was defined as indeterminate.

* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology, The University of Sydney at Westmead Hospital, Rm. 3114, Level 3, ICPMR, Westmead Hospital, Darcy Rd., Westmead, New South Wales 2145, Australia. Phone: 61-2-9845-6012. Fax: 61-2-9891-5317. E-mail: tanias{at}icpmr.wsahs.nsw.gov.au.

Journal of Clinical Microbiology, August 2001, p. 2916-2923, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2916-2923.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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