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Journal of Clinical Microbiology, August 2001, p. 2951-2953, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2951-2953.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cyclospora cayetanensis in Three Populations at Risk in Guatemala

Rafael A. Pratdesaba,1,* Mario González,2 Evelyn Piedrasanta,1,2 Claudia Mérida,1,2 Karen Contreras,1,2 Carlos Vela,3 Francisco Culajay,1 Luis Flores,4 and Olga Torres1

Laboratorios de Microbiología y Virología, Instituto de Nutrición de Centro América y Panamá (INCAP),1 Escuela de Química Biológica, Facultad de Ciencias Químicas y Farmacia, Universidad de San Carlos de Guatemala,2 Facultad de Medicina, Universidad Francisco Marroquín,3 and Programa Integral de Protección Agrícola y Ambiental (PIPAA), Ministerio de Agricultura Ganadería y Alimentación (MAGA) de Guatemala and Asociación Gremial de Exportadores de Productos no Tradicionales (AGEXPRONT),4 Guatemala City, Guatemala

Received 15 February 2001/Returned for modification 28 March 2001/Accepted 7 May 2001

In 1996 and 1997, outbreaks of Cyclospora cayetanensis in North America were linked to Guatemalan raspberries. From April 1999 to April 2000, we undertook a survey for C. cayetanensis in raspberry farm workers, malnourished children, and human immunodeficiency virus and AIDS patients in Guatemala. Stool samples were analyzed using ethylacetate-formalin concentration, wet preparation, modified acid-fast staining method, and epifluorescence. Oocysts were found in 1.5% of the subjects, none of whom were raspberry farm workers.

* Corresponding author. Mailing address: Laboratorios de Microbiología y Virología, Instituto de Nutrición de Centro América y Panamá (INCAP), Calzada Roosevelt, Zona 11 Apartado Postal 1188, Guatemala 01011, Guatemala. Phone: (502)-440-9802. Fax: (502)-473-6529. E-mail: rpratdes{at}incap.org.gt.

Journal of Clinical Microbiology, August 2001, p. 2951-2953, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2951-2953.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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