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Journal of Clinical Microbiology, August 2001, p. 2984-2986, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2984-2986.2001

Simplified Microneutralization Test for Serotyping Adenovirus Isolates

Marietta D. Malasig,1,* Pulak R. Goswami,1,dagger Leta K. Crawford-Miksza,2 David P. Schnurr,2 and Gregory C. Gray1

Department of Defense Center for Deployment Health Research, Naval Health Research Center, San Diego,1 and Viral and Rickettsial Disease Laboratory, California Department of Health Services, Richmond,2 California

Received 18 December 2000/Returned for modification 7 March 2001/Accepted 7 May 2001

A simplified microneutralization procedure is described that uses an empirically determined virus challenge dose, a single dilution of antiserum, and observation of cytopathic effect to determine the adenovirus serotype. The simplified test has faster turnaround time and was 96% concordant with a confirmatory test using serial dilutions of type-specific sera. This method will find utility in high-volume serotyping work.

* Corresponding author. Mailing address: DoD Center for Deployment Health Research, Naval Health Research Center, P.O. Box 85122, San Diego, CA 92186. Phone: (619) 553-8771. Fax: (619) 553-8449. E-mail: malasig{at}nhrc.navy.mil.

dagger Present address: Amsterdam Medical Center, University of Amsterdam, The Netherlands.

Journal of Clinical Microbiology, August 2001, p. 2984-2986, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.2984-2986.2001


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