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Journal of Clinical Microbiology, August 2001, p. 3006-3008, Vol. 39, No. 8
Departments of Laboratory Medicine and
Pathology and Pediatrics, University of Minnesota Medical
School, Minneapolis, Minnesota 55455
Received 22 February 2001/Returned for modification 11 April
2001/Accepted 28 May 2001
We developed a rapid pulsed-field gel electrophoresis (PFGE) method
that required 3 days to complete, an improvement over the standard
method that required as many as 8 days. The accuracy and
reproducibility of the rapid method were verified by analysis of DNA
band sizes of our control group B streptococcus isolate. The rapid
method was superior to the standard method, providing more precise
molecular sizing and gels of higher image quality. The reproducibility
of rapid PFGE substantiated its value and continued use.
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.8.3006-3008.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.
Rapid Pulsed-Field Gel Electrophoresis Method
for Group B Streptococcus Isolates
*
Corresponding author. Mailing address: University of
Minnesota Medical School, 420 Delaware St. SE, Mayo Mail Code 134, Minneapolis, MN 55455. Phone: (612) 624-1948. Fax: (612) 624-8927. E-mail: ferri002{at}maroon.tc.umn.edu.
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