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Journal of Clinical Microbiology, August 2001, p. 3006-3008, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.3006-3008.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Rapid Pulsed-Field Gel Electrophoresis Method for Group B Streptococcus Isolates

Jeffrey A. Benson and Patricia Ferrieri*

Departments of Laboratory Medicine and Pathology and Pediatrics, University of Minnesota Medical School, Minneapolis, Minnesota 55455

Received 22 February 2001/Returned for modification 11 April 2001/Accepted 28 May 2001

We developed a rapid pulsed-field gel electrophoresis (PFGE) method that required 3 days to complete, an improvement over the standard method that required as many as 8 days. The accuracy and reproducibility of the rapid method were verified by analysis of DNA band sizes of our control group B streptococcus isolate. The rapid method was superior to the standard method, providing more precise molecular sizing and gels of higher image quality. The reproducibility of rapid PFGE substantiated its value and continued use.

* Corresponding author. Mailing address: University of Minnesota Medical School, 420 Delaware St. SE, Mayo Mail Code 134, Minneapolis, MN 55455. Phone: (612) 624-1948. Fax: (612) 624-8927. E-mail: ferri002{at}maroon.tc.umn.edu.

Journal of Clinical Microbiology, August 2001, p. 3006-3008, Vol. 39, No. 8
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.8.3006-3008.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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