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Journal of Clinical Microbiology, September 2001, p. 3056-3059, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3056-3059.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

LightCycler Multiplex PCR for the Laboratory Diagnosis of Common Viral Infections of the Central Nervous System

Steven J. Read,* Joanne L. Mitchell, and Colin G. Fink

Micropathology Ltd., University of Warwick Science Park, Coventry CV4 7EZ, United Kingdom

Received 12 February 2001/Returned for modification 10 April 2001/Accepted 11 June 2001

A conventional multiplex PCR assay that detects herpes simplex virus type 1 (HSV-1), HSV-2, varicella-zoster virus, and enteroviruses for the diagnosis of central nervous system infections was modified to be performed using the LightCycler system. The sensitivity of detection of each of the viruses using the LightCycler assay was compared to that of the conventional assay using external quality assessment material. The assays had equivalent sensitivities, but the LightCycler assay was more rapid, reduced the risk of contamination, and used an amplicon detection format that demonstrated greater discrimination than a gel electrophoresis method.

* Corresponding author. Mailing address: Micropathology Ltd., University of Warwick Science Park, Barclays Venture Centre, Sir William Lyons Rd., Coventry CV4 7EZ, United Kingdom. Phone: 44-(0)2476-323222. Fax: 44-(0)2476-323333. E-mail: sjread{at}micropathology.com.

Journal of Clinical Microbiology, September 2001, p. 3056-3059, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3056-3059.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2001 by the American Society for Microbiology. All rights reserved.