Seven Human Immunodeficiency Virus (HIV) Antigen-Antibody Combination Assays: Evaluation of HIV Seroconversion Sensitivity and Subtype Detection

doi:10.1128/JCM.39.9.3122-3128.2001

This Article

	Full Text
	Full Text (PDF)
	Alert me when this article is cited
	Alert me if a correction is posted

Services

	Similar articles in this journal
	Similar articles in PubMed
	Alert me to new issues of the journal
	Download to citation manager
	Reprints and Permissions
	Copyright Information
	Books from ASM Press
	MicrobeWorld

Citing Articles

	Citing Articles via HighWire
	Citing Articles via Google Scholar

Google Scholar

	Articles by Ly, T. D.
	Articles by Devare, S. G.
	Search for Related Content

PubMed

	PubMed Citation
	Articles by Ly, T. D.
	Articles by Devare, S. G.

Previous Article | Next Article

Journal of Clinical Microbiology, September 2001, p. 3122-3128, Vol. 39, No. 9
0095-1137/01/$04.00+0 DOI: 10.1128/JCM.39.9.3122-3128.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Seven Human Immunodeficiency Virus (HIV) Antigen-Antibody Combination Assays: Evaluation of HIV Seroconversion Sensitivity and Subtype Detection

Thoai Duong Ly,¹ Lynn Martin,² David Daghfal,² Arnold Sandridge,² Daniel West,² Richard Bristow,³ Laurence Chalouas,¹ Xiaoxing Qiu,² Sheng C. Lou,² Jeffrey C. Hunt,² Gerald Schochetman,² and Sushil G. Devare²^,^*

Laboratoire Claude Levy, Ivry sur Seine, France¹; Abbott Laboratories, Diagnostics Division, Abbott Park, Illinois²; and Murex Biotech Ltd., Dartford, Kent, United Kingdom³

Received 5 February 2001/Returned for modification 13 April 2001/Accepted 18 June 2001

In this study, we evaluated the performance of two prototype human immunodeficiency virus (HIV) antigen-antibody (Ag-Ab) combinationassays, one from Abbott Laboratories (AxSYM HIV Ag-Ab) and theother from bioMerieux (VIDAS HIV Duo Ultra), versus five combinationassays commercially available in Europe. The assays were EnzygnostHIV Integral, Genscreen Plus HIV Ag-Ab, Murex HIV Ag-Ab Combination,VIDAS HIV Duo, and Vironostika HIV Uniform II Ag-Ab. All assayswere evaluated for the ability to detect p24 antigen from HIV-1groups M and O, antibody-positive plasma samples from HIV-1 groupsM and O, HIV-2, and 19 HIV seroconversion panels. Results indicatethat although all combination assays can detect antibodies toHIV-1, group M, subtypes A to G, circulating recombinant form(CRF) A/E, and HIV-1 group O, their sensitivity varied considerablywhen tested using diluted HIV-1 group O and HIV-2 antibody-positivesamples. Among combination assays, the AxSYM, Murex, and VIDASHIV Duo Ultra assays exhibited the best antigen sensitivity (at~25 pg of HIV Ag/ml) for detection of HIV-1 group M, subtypesA to G and CRF A/E, and HIV-1 group O isolates. However, the VIDASHIV Duo Ultra assay had a lower sensitivity for HIV-1 group Mand subtype C, and was unable to detect subtype C antigen evenat 125 pg of HIV Ag/ml. The HIV antigen sensitivity of the VIDASHIV Duo and Genscreen Plus combination assays was ~125 pg of HIVAg/ml for detection of all HIV-1 group M isolates except HIV-1group O while the sensitivity of Vironostika HIV Uniform II Ag-Aband Enzygnost HIV Integral Ag-Ab assays for all the group M subtypeswas >125 pg of HIV Ag/ml. Among the combination assays, the AxSYMassay had the best performance for detection of early seroconversionsamples, followed by the Murex and VIDAS HIV Duo Ultraassays.

^* Corresponding author. Mailing address: AIDS Research and Retrovirus Discovery, Abbott Laboratories, D-09NG, Bldg. AP 20, 100Abbott Park Rd., Abbott Park, IL 60064-6015. Phone: (847) 937-0913.Fax: (847) 937-1401. E-mail: Sushil.Devare{at}abbott.com.

This article has been cited by other articles:

Guan, M. (2007). Frequency, Causes, and New Challenges of Indeterminate Results in Western Blot Confirmatory Testing for Antibodies to Human Immunodeficiency Virus. CVI 14: 649-659 [Full Text]
Marcelino, J. M., Barroso, H., Goncalves, F., Silva, S. M., Novo, C., Gomes, P., Camacho, R., Taveira, N. (2006). Use of a New Dual-Antigen Enzyme-Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins. J. Clin. Microbiol. 44: 607-611 [Abstract] [Full Text]
Iqbal, H. S., Solomon, S., Murugavel, K. G., Solomon, S. S., Balakrishnan, P. (2005). Evaluation and Diagnostic Usefulness of Domestic and Imported Enzyme-Linked Immunosorbent Assays for Detection of Human Immunodeficiency Virus Type 1 Antibody in India. CVI 12: 1425-1428 [Abstract] [Full Text]
Gaudy, C., Moreau, A., Brunet, S., Descamps, J.-M., Deleplanque, P., Brand, D., Barin, F. (2004). Subtype B Human Immunodeficiency Virus (HIV) Type 1 Mutant That Escapes Detection in a Fourth-Generation Immunoassay for HIV Infection. J. Clin. Microbiol. 42: 2847-2849 [Abstract] [Full Text]
Sickinger, E., Stieler, M., Kaufman, B., Kapprell, H.-P., West, D., Sandridge, A., Devare, S., Schochetman, G., Hunt, J. C., Daghfal, D. (2004). Multicenter Evaluation of a New, Automated Enzyme-Linked Immunoassay for Detection of Human Immunodeficiency Virus-Specific Antibodies and Antigen. J. Clin. Microbiol. 42: 21-29 [Abstract] [Full Text]
Weber, B., Ly, T. D., Hunt, J., Devare, S. G. (2002). Human Immunodeficiency Virus (HIV) Antigen-Antibody Combination Assays: Evaluation of HIV Seroconversion Sensitivity and Subtype Detection. J. Clin. Microbiol. 40: 4402-4404 [Full Text]
Weber, B., Gurtler, L., Thorstensson, R., Michl, U., Muhlbacher, A., Burgisser, P., Villaescusa, R., Eiras, A., Gabriel, C., Stekel, H., Tanprasert, S., Oota, S., Silvestre, M.-J., Marques, C., Ladeira, M., Rabenau, H., Berger, A., Schmitt, U., Melchior, W. (2002). Multicenter Evaluation of a New Automated Fourth-Generation Human Immunodeficiency Virus Screening Assay with a Sensitive Antigen Detection Module and High Specificity. J. Clin. Microbiol. 40: 1938-1946 [Abstract] [Full Text]
Weber, B., Berger, A., Rabenau, H., Doerr, H. W. (2002). Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay, VIDAS HIV DUO Ultra. J. Clin. Microbiol. 40: 1420-1426 [Abstract] [Full Text]

Antimicrob. Agents Chemother.	Clin. Microbiol. Rev.

Clin. Vaccine Immunol.	ALL ASM JOURNALS