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Journal of Clinical Microbiology, September 2001, p. 3156-3163, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3156-3163.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Enzyme-Linked Immunosorbent Assay for Detecting Herpesvirus Exposure in Mediterranean Tortoises (Spur-Thighed Tortoise [Testudo graeca] and Hermann's Tortoise [Testudo hermanni])†

F. C. Origgi,1,2,* P. A. Klein,3 K. Mathes,4 S. Blahak,5 R. E. Marschang,6 S. J. Tucker,1 and E. R. Jacobson1

Department of Small Animal Clinical Sciences,1 Department of Pathobiology,2 and Department of Pathology,3 University of Florida, Gainesville, Florida 32610-0126, and Klinik fuer kleine Haustiere Tieraerztliche Hochschule, Hannover,4 Staatliches Veterinaruntersuchungsamt, Detmold,5 and Institute for Avian and Reptile Medicine, Justus Liebig University, Giessen,6 Germany

Received 20 November 2000/Returned for modification 8 April 2001/Accepted 17 June 2001

An enzyme-linked immunosorbent assay (ELISA) was developed for the detection of antibodies to a herpesvirus associated with an upper respiratory tract disease in Mediterranean tortoises [spur-thighed tortoise (Testudo graeca) and Hermann's tortoise (Testudo hermanni)]. This serodiagnostic test was validated through a hyperimmunization study. The mean of the A405 readings of the plasma samples collected at time zero of the hyperimmunization study plus three times the standard deviation was used as the cutoff for seropositivity in tortoises. ELISA results were compared to serum neutralization (SN) values for the same samples by using the McNemar test. The results obtained by SN and ELISA were not significantly different (P > 0.05). This new ELISA could be used as an important diagnostic tool for screening wild populations and private and zoo collections of Mediterranean tortoises.

* Corresponding author. Mailing address: Departments of Small Animal Clinical Sciences and Pathobiology, College of Veterinary Medicine, University of Florida, Gainesville, FL 32610-0126. Phone: (352) 392-4700, ext. 5252. Fax: (352) 392-6125. E-mail: origgif{at}mail.vetmed.ufl.edu.

dagger College of Veterinary Medicine Journal Series number 586.

Journal of Clinical Microbiology, September 2001, p. 3156-3163, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3156-3163.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Antimicrob. Agents Chemother. Clin. Microbiol. Rev.
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Copyright © 2001 by the American Society for Microbiology. All rights reserved.