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Journal of Clinical Microbiology, September 2001, p. 3350-3352, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3350-3352.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Detection of rpoB Mutations in Mycobacterium tuberculosis by Biprobe Analysis

K. J. Edwards,1 L. A. Metherell,1 M. Yates,2 and N. A. Saunders1,*

Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, Colindale, London, NW9 5HT,1 and PHLS Mycobacterium Reference Unit, Dulwich Public Health Laboratory, Dulwich Hospital, London, SE22 8QF,2 United Kingdom

Received 16 April 2001/Returned for modification 4 May 2001/Accepted 27 June 2001

A biprobe assay utilizing LightCycler technology was developed to detect rifampin resistance-associated gene mutations in the Mycobacterium tuberculosis rpoB gene. Three biprobes detected all mutations present in the 46 rifampin-resistant isolates. Wild-type sequences were correctly identified in each case. The method was reproducible, accurate, and easy to use.

* Corresponding author. Mailing address: Molecular Biology Unit, Virus Reference Division, Central Public Health Laboratory, 61 Colindale Ave., Colindale, London, NW9 5HT, United Kingdom. Phone: 020 8200 4400, ext. 3070. Fax: 020 8200 1569. E-mail: nsaunders{at}phls.org.uk.

Journal of Clinical Microbiology, September 2001, p. 3350-3352, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3350-3352.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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