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Journal of Clinical Microbiology, September 2001, p. 3368-3372, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3368-3372.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Cysticercus Antigens in Cerebrospinal Fluid Samples from Patients with Neurocysticercosis

Alessandra Xavier Pardini,1 Adelaide José Vaz,1,* Luis Dos Ramos Machado,2 and José Antônio Livramento2

Laboratory of Clinical Immunology, Faculty of Pharmaceutical Sciences, 05508-900 São Paulo,1 and Center of Neurological Investigation, Faculty of Medicine, University of São Paulo, 01246-903 São Paulo,2 SP, Brazil

Received 27 January 2001/Returned for modification 3 April 2001/Accepted 6 July 2001

Antigens were detected in cerebrospinal fluid (CSF) samples from patients with neurocysticercosis (NC) by enzyme-linked immunosorbent assay (ELISA) using polyclonal sera of rabbit anti-Taenia solium cysticerci (anti-Tso) and anti- Taenia crassiceps cysticerci vesicular fluid (anti-Tcra or anti-Tcra <30 kDa). A group of NC patients (n = 174) were studied (NC), including 40 patients in different phases of the disease. ELISAs carried out with the anti-Tso, anti-Tcra, and anti-Tcra <30 kDa showed sensitivities of 81.2, 90, and 95.8% and specificities of 82, 98, and 100%, respectively. The 14- and 18-kDa low-molecular-weight peptides were only detected in CSF samples from patients with NC by immunoblotting with anti-Tso and anti-Tcra sera. Because of the importance of the diagnosis and prognosis of cysticercosis, the detection of antigens may contribute as an additional marker to the study and clarification of the parasite-host relationship.

* Corresponding author. Mailing address: Faculdade de Ciências Farmacêuticas-Universidade de São Paulo, Av. Lineu Prestes, 580, Bloco 17, Laboratório de Imunologia Clínica, CEP 05508-900, São Paulo, SP, Brazil. Phone: 55-11-38183638. Fax: 55-11-38132197. E-mail: ajvaz{at}netpoint.com.br or pardini{at}usp.br.

Journal of Clinical Microbiology, September 2001, p. 3368-3372, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3368-3372.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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