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Journal of Clinical Microbiology, September 2001, p. 3382-3385, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3382-3385.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Application of PCR to Distinguish Common Species of Dermatophytes

Elisabetta Faggi,1,* Gabriella Pini,1 Enza Campisi,1 Chiara Bertellini,1 Elisa Difonzo,2 and Francesca Mancianti3

Dipartimento di Sanità Pubblica---Sezione Microbiologia, Università di Firenze, 50134 Florence,1 Dipartimento di Scienze Dermatologiche, Università di Firenze, 50121 Florence,2 and Dipartimento di Patologia Animale, Università di Pisa, 56124 Pisa,3 Italy

Received 30 March 2001/Returned for modification 16 May 2001/Accepted 8 July 2001

This report describes the application of PCR fingerprinting for the identification of species and varieties of common dermatophytes and related fungi utilizing as a single primer the simple repetitive oligonucleotide (GACA)4. The primer was able to amplify all the strains, producing species-specific profiles for Microsporum canis, Microsporum gypseum, Trichophyton rubrum, Trichophyton ajelloi, and Epidermophyton floccosum. Intraspecific variability was not observed for these species. Instead, three different profiles were observed in the Trichophyton mentagrophytes group.

* Corresponding author. Mailing address: Dipartimento di Sanità Pubblica---Sezione Microbiologia, Viale Morgagni 48, 50134 Florence, Italy. Phone: (055) 411081. Fax: (055) 4223895. E-mail: efaggi{at}unifi.it.

Journal of Clinical Microbiology, September 2001, p. 3382-3385, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3382-3385.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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