This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Vliet, K. E.
Right arrow Articles by Van Loon, A. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Vliet, K. E.
Right arrow Articles by Van Loon, A. M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, September 2001, p. 3390-3392, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3390-3392.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multicenter Proficiency Testing of Nucleic Acid Amplification Methods for the Detection of Enteroviruses

Karin E. Van Vliet,1,dagger Peter Muir,2,dagger Jose M. Echevarria,3,dagger Paul E. Klapper,4,dagger Graham M. Cleator,5,dagger and Anton M. Van Loon1,*,dagger

Department of Virology, University Medical Center Utrecht, Utrecht, The Netherlands1; C.N.M., Instituto de Salud Carlos III, Madrid, Spain3; and King's College London, London,2 Public Health Laboratory, Leeds,4 and Manchester Royal Infirmary, Manchester,5 United Kingdom

Received 1 December 2000/Returned for modification 18 April 2001/Accepted 11 July 2001

A multicenter study of molecular detection of enteroviruses was conducted using a proficiency panel. Of 70 data sets, 46 (66%) reported correct results for samples containing at least 1 50% infective dose per ml and for negative samples. Variation in performance between laboratories demonstrates the need for ongoing quality control.

* Corresponding author. Mailing address: University Medical Center, Department of Virology G04.614, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. Phone: 31 (0) 30 2507629. Fax: 31 (0) 30 2505426. E-mail: a.m.vanloon{at}lab.azu.nl.

dagger Member of the European Union Concerted Action on Quality Control of Nucleic Acid Amplification in Diagnostic Virology.

Journal of Clinical Microbiology, September 2001, p. 3390-3392, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3390-3392.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


This article has been cited by other articles:

  • Kost, C. B., Rogers, B., Oberste, M. S., Robinson, C., Eaves, B. L., Leos, K., Danielson, S., Satya, M., Weir, F., Nolte, F. S. (2007). Multicenter Beta Trial of the GeneXpert Enterovirus Assay. J. Clin. Microbiol. 45: 1081-1086 [Abstract] [Full Text]  
  • Hietala, S. K., Crossley, B. M. (2006). Armored RNA as Virus Surrogate in a Real-Time Reverse Transcriptase PCR Assay Proficiency Panel. J. Clin. Microbiol. 44: 67-70 [Abstract] [Full Text]  
  • Davies, N W S, Brown, L J, Gonde, J, Irish, D, Robinson, R O, Swan, A V, Banatvala, J, Howard, R S, Sharief, M K, Muir, P (2005). Factors influencing PCR detection of viruses in cerebrospinal fluid of patients with suspected CNS infections. J. Neurol. Neurosurg. Psychiatry 76: 82-87 [Abstract] [Full Text]  
  • Niedrig, M., Schmitz, H., Becker, S., Gunther, S., ter Meulen, J., Meyer, H., Ellerbrok, H., Nitsche, A., Gelderblom, H. R., Drosten, C. (2004). First International Quality Assurance Study on the Rapid Detection of Viral Agents of Bioterrorism. J. Clin. Microbiol. 42: 1753-1755 [Abstract] [Full Text]  
  • Verkooyen, R. P., Noordhoek, G. T., Klapper, P. E., Reid, J., Schirm, J., Cleator, G. M., Ieven, M., Hoddevik, G. (2003). Reliability of Nucleic Acid Amplification Methods for Detection of Chlamydia trachomatis in Urine: Results of the First International Collaborative Quality Control Study among 96 Laboratories. J. Clin. Microbiol. 41: 3013-3016 [Abstract] [Full Text]  
  • Schirm, J., van Loon, A. M., Valentine-Thon, E., Klapper, P. E., Reid, J., Cleator, G. M. (2002). External Quality Assessment Program for Qualitative and Quantitative Detection of Hepatitis C Virus RNA in Diagnostic Virology. J. Clin. Microbiol. 40: 2973-2980 [Abstract] [Full Text]  
  • Valentine-Thon, E., van Loon, A. M., Schirm, J., Reid, J., Klapper, P. E., Cleator, G. M. (2001). European Proficiency Testing Program for Molecular Detection and Quantitation of Hepatitis B Virus DNA. J. Clin. Microbiol. 39: 4407-4412 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»