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Journal of Clinical Microbiology, September 2001, p. 3390-3392, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3390-3392.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Multicenter Proficiency Testing of Nucleic Acid Amplification Methods for the Detection of Enteroviruses

Karin E. Van Vliet,1,dagger Peter Muir,2,dagger Jose M. Echevarria,3,dagger Paul E. Klapper,4,dagger Graham M. Cleator,5,dagger and Anton M. Van Loon1,*,dagger

Department of Virology, University Medical Center Utrecht, Utrecht, The Netherlands1; C.N.M., Instituto de Salud Carlos III, Madrid, Spain3; and King's College London, London,2 Public Health Laboratory, Leeds,4 and Manchester Royal Infirmary, Manchester,5 United Kingdom

Received 1 December 2000/Returned for modification 18 April 2001/Accepted 11 July 2001

A multicenter study of molecular detection of enteroviruses was conducted using a proficiency panel. Of 70 data sets, 46 (66%) reported correct results for samples containing at least 1 50% infective dose per ml and for negative samples. Variation in performance between laboratories demonstrates the need for ongoing quality control.

* Corresponding author. Mailing address: University Medical Center, Department of Virology G04.614, Heidelberglaan 100, 3584 CX Utrecht, The Netherlands. Phone: 31 (0) 30 2507629. Fax: 31 (0) 30 2505426. E-mail: a.m.vanloon{at}lab.azu.nl.

dagger Member of the European Union Concerted Action on Quality Control of Nucleic Acid Amplification in Diagnostic Virology.

Journal of Clinical Microbiology, September 2001, p. 3390-3392, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3390-3392.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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Copyright © 2001 by the American Society for Microbiology. All rights reserved.