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Journal of Clinical Microbiology, September 2001, p. 3398-3401, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3398-3401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.

Molecular Typing of the Etiologic Agent of Human Granulocytic Ehrlichiosis

Sarah E. Carter, M. Dana Ravyn, Yaning Xu, and Russell C. Johnson*

Department of Microbiology, University of Minnesota Academic Health Center, Minneapolis, Minnesota 55455

Received 15 March 2001/Returned for modification 1 June 2001/Accepted 1 July 2001

The p44 gene of the agent of human granulocytic ehrlichiosis (aoHGE) encodes a 44-kDa major outer surface protein. A technique was developed for the typing of the aoHGE based on the PCR amplification of the p44 gene followed by a multiple restriction digest with HindIII, EcoRV, and AspI to generate restriction fragment length polymorphism patterns. Twenty-four samples of the aoHGE were collected from geographically dispersed sites in the United States and included isolates from humans, equines, canines, small mammals, and ticks. Six granulocytic ehrlichiosis (GE) types were identified. The GE typing method is relatively simple to perform, is reproducible, and is able to differentiate among the various isolates of granulocytic ehrlichiae in the United States. These characteristics suggest that this GE typing method may be an important epizootiological and epidemiological tool.

* Corresponding author. Mailing address: University of Minnesota Academic Health Center, 420 Delaware St. SE, Mayo Mail Code 196, Minneapolis, MN 55455-0312. Phone: (612) 624-7944. Fax: (612) 626-0623. E-mail: johnson{at}mail.ahc.umn.edu.

Journal of Clinical Microbiology, September 2001, p. 3398-3401, Vol. 39, No. 9
0095-1137/01/$04.00+0   DOI: 10.1128/JCM.39.9.3398-3401.2001
Copyright © 2001, American Society for Microbiology. All rights reserved.


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