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Journal of Clinical Microbiology, January 2002, p. 146-149, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.146-149.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Genomic Diversity of Group A Rotavirus Strains Infecting Humans in Eastern India

Soma Das,1 A. Sen,1 G. Uma,1 V. Varghese,1 S. Chaudhuri,1 S. K. Bhattacharya,2,3 T. Krishnan,1 P. Dutta,2 D. Dutta,2 M. K. Bhattacharya,2 U. Mitra,2 N. Kobayashi,4 and T. N. Naik1,3*

Division of Virology,1 Division of Clinical Medicine,2 ICMR Virus Unit, GB-4, ID and BG Hospital Campus,3 Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060, Japan4

Received 30 May 2001/ Returned for modification 20 July 2001/ Accepted 15 October 2001

Between 1998 and 2000, a total of 266 samples were found positive for group A rotaviruses by RNA electrophoresis. Samples were collected from patients admitted to two leading hospitals in Calcutta. Serotyping could be done only with 22% of the positive samples, leaving 78% untypeable. The G (VP7 genotypes) and P (VP4 genotypes) types were determined for 159 samples by reverse transcription and multiplex PCR. The predominant genotype was G1P[8] (20%), followed by G2P[4] (15%) and G4P[8] (6%). A number of uncommon genotypes, G1P[4] (4%), G2P[8] (2.5%), G2P[6] (0.6%), G4P[4] (2.5%), and G4P[6] (1.25%), were also detected during this study period. Twenty two percent of specimens showed mixed infections, 38 (24%) of the total samples remained untypeable for either VP7 or VP4, while only 4 (2.5%) of the samples were untypeable for both genes. Eleven specimens collected from Manipur were also genotyped and revealed a very high degree of genomic reassortment.

* Corresponding author. Mailing address: Division of Virology, National Institute of Cholera and Enteric Diseases, P-33 CIT Rd., Scheme XM, Beliaghata, Kolkata (Calcutta) 700010, India. Phone: 91-33-350 0448. Fax: 91-33-350 5066. E-mail: tnaik{at}satyam.net.in.

Journal of Clinical Microbiology, January 2002, p. 146-149, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.146-149.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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