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Journal of Clinical Microbiology, January 2002, p. 150-154, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.150-154.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Multicenter Evaluation of Fully Automated BACTEC Mycobacteria Growth Indicator Tube 960 System for Susceptibility Testing of Mycobacterium tuberculosis

Pascale Bemer,1* Frantiska Palicova,2 Sabine Rüsch-Gerdes,3 Henri B. Drugeon,1 and Gaby E. Pfyffer2

Department of Microbiology, Laënnec University Hospital, Nantes, France,1 Swiss National Center for Mycobacteria, Department of Medical Microbiology, Zurich, Switzerland,2 National Reference Center for Mycobacteria, Borstel, Germany3

Received 22 June 2001/ Returned for modification 5 August 2001/ Accepted 10 September 2001

The reliability of the BACTEC Mycobacteria Growth Indicator Tube (MGIT) 960 system for testing of Mycobacterium tuberculosis susceptibility to the three front-line drugs (isoniazid [INH], rifampin [RIF], and ethambutol [EMB]) plus streptomycin (STR) was compared to that of the BACTEC 460 TB system. The proportion method was used to resolve discrepant results by an independent arbiter. One hundred and ten strains were tested with an overall agreement of 93.5%. Discrepant results were obtained for seven strains (6.4%) with INH (resistant by BACTEC MGIT 960; susceptible by BACTEC 460 TB), for one strain (0.9%) with RIF (resistant by BACTEC MGIT 960; susceptible by BACTEC 460 TB), for seven strains (6.4%) with EMB (six resistant by BACTEC MGIT 960 and susceptible by BACTEC 460 TB; one susceptible by BACTEC MGIT 960 and resistant by BACTEC 460 TB), and for 19 strains (17.3%) with STR (resistant by BACTEC MGIT 960 and susceptible by BACTEC 460 TB). After resolution of discrepant results, the sensitivity of the BACTEC MGIT 960 system was 100% for all four drugs and specificity ranged from 89.8% for STR to 100% for RIF. Turnaround times were 4.6 to 11.7 days (median, 6.5 days) for BACTEC MGIT 960 and 4.0 to 10.0 days (median, 7.0 days) for BACTEC 460 TB. These data demonstrate that the fully automated and nonradiometric BACTEC MGIT 960 system is an accurate method for rapid susceptibility testing of M. tuberculosis.

* Corresponding author. Mailing address: Department of Microbiology, Laënnec University Hospital, Bd J. Monod, 44093 Saint-Herblain cedex 1, France. Phone: (33) 2 40 16 54 57. Fax: (33) 2 40 16 54 55. E-mail: pascale.bemer{at}chu-nantes.fr.

Journal of Clinical Microbiology, January 2002, p. 150-154, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.150-154.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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