This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kong, F.
Right arrow Articles by Gilbert, G. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2002, p. 216-226, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.216-226.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Serotype Identification of Group B Streptococci by PCR and Sequencing

Fanrong Kong,1 Sonia Gowan,2 Diana Martin,2 Gregory James,1 and Gwendolyn L. Gilbert1*

Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead, New South Wales, Australia,1 Institute of Environmental Science and Research, Porirua, Wellington, New Zealand2

Received 19 June 2001/ Returned for modification 7 August 2001/ Accepted 7 October 2001

Group B streptococcus (GBS; Streptococcus agalactiae) is the most common cause of neonatal and obstetric sepsis and is an increasingly important cause of septicemia in elderly individuals and immunocompromised patients. Ongoing surveillance to monitor GBS serotype distribution will be needed to guide the development and use of GBS conjugate vaccines. We designed sequencing primers based on the previously published sequences of the capsular polysaccharide (cps) gene clusters to further define partial cps gene clusters for eight of the nine GBS serotypes (serotypes Ia to VII). Subsequently, we designed and evaluated primers to identify serotypes Ia, Ib, III, IV, V, and VI directly by PCR and all eight serotypes (serotypes Ia to VII) by sequence heterogeneity. A total of 206 clinical GBS isolates were used to compare our molecular serotype (MS) identification method with conventional serotyping (CS). All clinical isolates were assigned an MS, whereas 188 of 206 (91.3%) were assigned a serotype by use of antisera. A small number of isolates (serosubtypes III-3 and III-4) showed different serotype specificities between PCR and sequencing, but the PCR results correlated with those obtained by CS. The overall agreement between the MS identification method and CS for isolates for which results of both tests were available was 100% (188 of 188 isolates). The MS identification method is a specific and practical alternative to conventional GBS serotyping and will facilitate epidemiological studies.


* Corresponding author. Mailing address: Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, Darcy Rd., Westmead, New South Wales, 2145 Australia. Phone: (612) 9845 6255. Fax: (612) 9893 8659. E-mail: lyng{at}icpmr.wsahs.nsw.gov.au.


Journal of Clinical Microbiology, January 2002, p. 216-226, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.216-226.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.




This article has been cited by other articles:

  • Lartigue, M.-F., Hery-Arnaud, G., Haguenoer, E., Domelier, A.-S., Schmit, P.-O., van der Mee-Marquet, N., Lanotte, P., Mereghetti, L., Kostrzewa, M., Quentin, R. (2009). Identification of Streptococcus agalactiae Isolates from Various Phylogenetic Lineages by Matrix-Assisted Laser Desorption Ionization-Time of Flight Mass Spectrometry. J. Clin. Microbiol. 47: 2284-2287 [Abstract] [Full Text]  
  • Ulett, K. B., Benjamin, W. H. Jr., Zhuo, F., Xiao, M., Kong, F., Gilbert, G. L., Schembri, M. A., Ulett, G. C. (2009). Diversity of Group B Streptococcus Serotypes Causing Urinary Tract Infection in Adults. J. Clin. Microbiol. 47: 2055-2060 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Jouannet, C., Domelier, A.-S., Arnault, L., Lartigue, M.-F., Quentin, R. (2009). Genetic diversity of Streptococcus agalactiae strains and density of vaginal carriage. J Med Microbiol 58: 169-173 [Abstract] [Full Text]  
  • Domelier, A.-S., van der Mee-Marquet, N., Arnault, L., Mereghetti, L., Lanotte, P., Rosenau, A., Lartigue, M.-F., Quentin, R. (2008). Molecular characterization of erythromycin-resistant Streptococcus agalactiae strains. J Antimicrob Chemother 62: 1227-1233 [Abstract] [Full Text]  
  • van der Mee-Marquet, N., Fourny, L., Arnault, L., Domelier, A.-S., Salloum, M., Lartigue, M.-F., Quentin, R. (2008). Molecular Characterization of Human-Colonizing Streptococcus agalactiae Strains Isolated from Throat, Skin, Anal Margin, and Genital Body Sites. J. Clin. Microbiol. 46: 2906-2911 [Abstract] [Full Text]  
  • Mavenyengwa, R. T., Maeland, J. A., Moyo, S. R. (2008). Distinctive Features of Surface-Anchored Proteins of Streptococcus agalactiae Strains from Zimbabwe Revealed by PCR and Dot Blotting. CVI 15: 1420-1424 [Abstract] [Full Text]  
  • Kong, F., Lambertsen, L. M., Slotved, H.-C., Ko, D., Wang, H., Gilbert, G. L. (2008). Use of Phenotypic and Molecular Serotype Identification Methods To Characterize Previously Nonserotypeable Group B Streptococci. J. Clin. Microbiol. 46: 2745-2750 [Abstract] [Full Text]  
  • Sigge, A., Schmid, M., Mauerer, S., Spellerberg, B. (2008). Heterogeneity of Hemolysin Expression during Neonatal Streptococcus agalactiae Sepsis. J. Clin. Microbiol. 46: 807-809 [Abstract] [Full Text]  
  • Martins, E. R., Florindo, C., Martins, F., Aldir, I., Borrego, M. J., Brum, L., Ramirez, M., Melo-Cristino, J. (2007). Streptococcus agalactiae Serotype Ib as an Agent of Meningitis in Two Adult Nonpregnant Women. J. Clin. Microbiol. 45: 3850-3852 [Abstract] [Full Text]  
  • Zhou, F., Kong, F., Tong, Z., Gilbert, G. L. (2007). Identification of Less-Common Streptococcus pneumoniae Serotypes by a Multiplex PCR-Based Reverse Line Blot Hybridization Assay. J. Clin. Microbiol. 45: 3411-3415 [Abstract] [Full Text]  
  • Cai, Y., Kong, F., Wang, Q., Tong, Z., Sintchenko, V., Zeng, X., Gilbert, G. L. (2007). Comparison of Single- and Multilocus Sequence Typing and Toxin Gene Profiling for Characterization of Methicillin-Resistant Staphylococcus aureus. J. Clin. Microbiol. 45: 3302-3308 [Abstract] [Full Text]  
  • Slotved, H.-C., Kong, F., Lambertsen, L., Sauer, S., Gilbert, G. L. (2007). Serotype IX, a Proposed New Streptococcus agalactiae Serotype. J. Clin. Microbiol. 45: 2929-2936 [Abstract] [Full Text]  
  • Gherardi, G., Imperi, M., Baldassarri, L., Pataracchia, M., Alfarone, G., Recchia, S., Orefici, G., Dicuonzo, G., Creti, R. (2007). Molecular Epidemiology and Distribution of Serotypes, Surface Proteins, and Antibiotic Resistance among Group B Streptococci in Italy. J. Clin. Microbiol. 45: 2909-2916 [Abstract] [Full Text]  
  • Poyart, C., Tazi, A., Reglier-Poupet, H., Billoet, A., Tavares, N., Raymond, J., Trieu-Cuot, P. (2007). Multiplex PCR Assay for Rapid and Accurate Capsular Typing of Group B Streptococci. J. Clin. Microbiol. 45: 1985-1988 [Abstract] [Full Text]  
  • Puopolo, K. M., Madoff, L. C. (2007). Type IV Neonatal Early-Onset Group B Streptococcal Disease in a United States Hospital. J. Clin. Microbiol. 45: 1360-1362 [Abstract] [Full Text]  
  • Ip, M., Cheuk, E. S. C., Tsui, M. H. Y., Kong, F., Leung, T. N., Gilbert, G. L. (2006). Identification of a Streptococcus agalactiae Serotype III Subtype 4 Clone in Association with Adult Invasive Disease in Hong Kong. J. Clin. Microbiol. 44: 4252-4254 [Abstract] [Full Text]  
  • Zeng, X., Kong, F., Morgan, J., Gilbert, G. L. (2006). Evaluation of a Multiplex PCR-Based Reverse Line Blot-Hybridization Assay for Identification of Serotype and Surface Protein Antigens of Streptococcus agalactiae.. J. Clin. Microbiol. 44: 3822-3825 [Abstract] [Full Text]  
  • Domelier, A.-S., van der Mee-Marquet, N., Grandet, A., Mereghetti, L., Rosenau, A., Quentin, R. (2006). Loss of Catabolic Function in Streptococcus agalactiae Strains and Its Association with Neonatal Meningitis.. J. Clin. Microbiol. 44: 3245-3250 [Abstract] [Full Text]  
  • Ramaswamy, S. V., Ferrieri, P., Flores, A. E., Paoletti, L. C. (2006). Molecular characterization of nontypeable group B streptococcus.. J. Clin. Microbiol. 44: 2398-2403 [Abstract] [Full Text]  
  • Kong, F., Gidding, H. F., Berner, R., Gilbert, G. L. (2006). Streptococcus agalactiae C{beta} protein gene (bac) sequence types, based on the repeated region of the cell-wall-spanning domain: relationship to virulence and a proposed standardized nomenclature.. J Med Microbiol 55: 829-837 [Abstract] [Full Text]  
  • Ramaswamy, S. V., Ferrieri, P., Madoff, L. C., Flores, A. E., Kumar, N., Tettelin, H., Paoletti, L. C. (2006). Identification of novel cps locus polymorphisms in nontypable group B Streptococcus. J Med Microbiol 55: 775-783 [Abstract] [Full Text]  
  • Kong, F., Brown, M., Sabananthan, A., Zeng, X., Gilbert, G. L. (2006). Multiplex PCR-Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes.. J. Clin. Microbiol. 44: 1887-1891 [Abstract] [Full Text]  
  • Wen, L., Wang, Q., Li, Y., Kong, F., Gilbert, G. L., Cao, B., Wang, L., Feng, L. (2006). Use of a Serotype-Specific DNA Microarray for Identification of Group B Streptococcus (Streptococcus agalactiae). J. Clin. Microbiol. 44: 1447-1452 [Abstract] [Full Text]  
  • Zeng, X., Kong, F., Wang, H., Darbar, A., Gilbert, G. L. (2006). Simultaneous Detection of Nine Antibiotic Resistance-Related Genes in Streptococcus agalactiae Using Multiplex PCR and Reverse Line Blot Hybridization Assay. Antimicrob. Agents Chemother. 50: 204-209 [Abstract] [Full Text]  
  • Zhao, Z., Kong, F., Gilbert, G. L. (2006). Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen Genes. CVI 13: 145-149 [Abstract] [Full Text]  
  • Dogan, B., Schukken, Y. H., Santisteban, C., Boor, K. J. (2005). Distribution of Serotypes and Antimicrobial Resistance Genes among Streptococcus agalactiae Isolates from Bovine and Human Hosts. J. Clin. Microbiol. 43: 5899-5906 [Abstract] [Full Text]  
  • Manning, S. D., Lacher, D. W., Davies, H. D., Foxman, B., Whittam, T. S. (2005). DNA Polymorphism and Molecular Subtyping of the Capsular Gene Cluster of Group B Streptococcus. J. Clin. Microbiol. 43: 6113-6116 [Abstract] [Full Text]  
  • Kong, F., Ma, L., Gilbert, G. L (2005). Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. J Med Microbiol 54: 1133-1138 [Abstract] [Full Text]  
  • Maeland, J. A., Bevanger, L., Lyng, R. V. (2005). Immunological Markers of the R4 Protein of Streptococcus agalactiae. CVI 12: 1305-1310 [Abstract] [Full Text]  
  • Sun, Y., Kong, F., Zhao, Z., Gilbert, G. L. (2005). Comparison of a 3-Set Genotyping System with Multilocus Sequence Typing for Streptococcus agalactiae (Group B Streptococcus). J. Clin. Microbiol. 43: 4704-4707 [Abstract] [Full Text]  
  • Hery-Arnaud, G., Bruant, G., Lanotte, P., Brun, S., Rosenau, A., van der Mee-Marquet, N., Quentin, R., Mereghetti, L. (2005). Acquisition of Insertion Sequences and the GBSi1 Intron by Streptococcus agalactiae Isolates Correlates with the Evolution of the Species. J. Bacteriol. 187: 6248-6252 [Abstract] [Full Text]  
  • Amundson, N. R., Flores, A. E., Hillier, S. L., Baker, C. J., Ferrieri, P. (2005). DNA Macrorestriction Analysis of Nontypeable Group B Streptococcal Isolates: Clonal Evolution of Nontypeable and Type V Isolates. J. Clin. Microbiol. 43: 572-576 [Abstract] [Full Text]  
  • Creti, R., Fabretti, F., Orefici, G., von Hunolstein, C. (2004). Multiplex PCR Assay for Direct Identification of Group B Streptococcal Alpha-Protein-Like Protein Genes. J. Clin. Microbiol. 42: 1326-1329 [Abstract] [Full Text]  
  • Borchardt, S. M., Foxman, B., Chaffin, D. O., Rubens, C. E., Tallman, P. A., Manning, S. D., Baker, C. J., Marrs, C. F. (2004). Comparison of DNA Dot Blot Hybridization and Lancefield Capillary Precipitin Methods for Group B Streptococcal Capsular Typing. J. Clin. Microbiol. 42: 146-150 [Abstract] [Full Text]  
  • Kong, F., Gilbert, G. L. (2003). Using cpsA-cpsB sequence polymorphisms and serotype-/group-specific PCR to predict 51 Streptococcus pneumoniae capsular serotypes. J Med Microbiol 52: 1047-1058 [Abstract] [Full Text]  
  • Ekelund, K., Slotved, H.-C., Nielsen, H. U., Kaltoft, M. S., Konradsen, H. B. (2003). Emergence of Invasive Serotype VIII Group B Streptococcal Infections in Denmark. J. Clin. Microbiol. 41: 4442-4444 [Abstract] [Full Text]  
  • Slotved, H.-C., Elliott, J., Thompson, T., Konradsen, H. B. (2003). Latex Assay for Serotyping of Group B Streptococcus Isolates. J. Clin. Microbiol. 41: 4445-4447 [Abstract] [Full Text]  
  • Jones, N., Bohnsack, J. F., Takahashi, S., Oliver, K. A., Chan, M.-S., Kunst, F., Glaser, P., Rusniok, C., Crook, D. W. M., Harding, R. M., Bisharat, N., Spratt, B. G. (2003). Multilocus Sequence Typing System for Group B Streptococcus. J. Clin. Microbiol. 41: 2530-2536 [Abstract] [Full Text]  
  • Kong, F., Martin, D., James, G., Gilbert, G. L. (2003). Towards a genotyping system for Streptococcus agalactiae (group B streptococcus): use of mobile genetic elements in Australasian invasive isolates. J Med Microbiol 52: 337-344 [Abstract] [Full Text]