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Journal of Clinical Microbiology, January 2002, p. 301-305, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.301-305.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Antibiotic-Resistant Cell-Detaching Escherichia coli Strains from Nigerian Children

Iruka N. Okeke,^1,2, Hartmut Steinrück,³ Kristen J. Kanack,¹ Simon J. Elliott,^1, Lars Sundström,⁴ James B. Kaper,^1* and Adebayo Lamikanra²

Department of Microbiology and Immunology and Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland 21201,¹ Department of Pharmaceutics, Obafemi Awolowo University, Ile-Ife, Nigeria,² Federal Institute for Health Protection of Consumers and Veterinary Medicine, D-12277 Berlin, Germany,³ Division of Microbiology, Department of Pharmaceutical Biosciences, Uppsala University, Uppsala S-751 23, Sweden⁴

Received 3 January 2001/ Returned for modification 10 February 2001/ Accepted 19 October 2001

The properties of 23 cell-detaching Escherichia coli strains that were isolated from stool specimens in Nigeria are described. Common properties of the strains included the presence of genes encoding -hemolysin (100%), pyelonephritis-associated pili (100%), and cytotoxic necrotizing factor 1 (70%) as well as lactose negativity (70%) and multiple antibiotic resistance (74%). Antibiotic resistance was shown in most cases to be transferable and associated with the presence of class 1 integrons. Phenotypic properties and pulsed-field gel electrophoresis analysis demonstrated that the majority of the strains, particularly multiply resistant, lactose-negative O4:H40 strains, were closely related. Multiply-resistant cell-detaching E. coli strains may represent an important reservoir for antibiotic resistance genes.

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* Corresponding author. Mailing address: Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, MD 21201. Phone: (410) 706-5328. Fax: (410) 706-0182. E-mail: jkaper{at}medicine.umaryland.edu.

Present address: Department of Biomedical Sciences, University of Bradford, Bradford, West Yorkshire, BD7 1DP, United Kingdom.

Present address: Johns Hopkins University Medical Institution, Baltimore, MD 21205.

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Journal of Clinical Microbiology, January 2002, p. 301-305, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.301-305.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

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