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Journal of Clinical Microbiology, January 2002, p. 44-51, Vol. 40, No. 1
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.1.44-51.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Immune Reactivity of Human Sera to the Glycoprotein B of Human Herpesvirus 7

Michael Franti,¹ Jean-Thierry Aubin,^1, Guillemette De Saint-Maur,² Haruhiko Kosuge,¹ Koichi Yamanishi,³ Agnes Gautheret-Dejean,¹ Antoine Garbarg-Chenon,² Jean-Marie Huraux,¹ and Henri Agut^1*

Laboratoire de Virologie, C.E.R.V.I., UPRES EA 2387, Hôpital Pitié-Salpétrière, 75651 Paris Cedex 13,¹ Laboratoire de Microbiologie, Unité de Virologie, Hôpital Armand Trousseau, 75571 Paris Cedex 12, France,² Department of Microbiology, Osaka University Medical School, Suita, Osaka, Japan³

Received 5 February 2001/ Returned for modification 12 May 2001/ Accepted 5 August 2001

The glycoprotein B (gB) is highly conserved among distinct human herpesvirus 7 (HHV-7) strains. Similarly to other herpesvirus glycoproteins, gB has been assumed to induce a specific human immune response. However, it did not appear as an immunodominant protein in conventional immunoblot assays. Recombinant gB, obtained from either Escherichia coli or baculovirus expression systems, did react specifically with HHV-7-seropositive sera, and the main corresponding epitopes were located in its N-terminal part. A 24-amino-acid peptide, corresponding to a predicted hydrophilicity peak and presenting no extensive homology with other betaherpesvirus glycoproteins, was selected in this region at positions 129 to 152 of the gB sequence. When tested by enzyme-linked immunosorbent assay (ELISA), this peptide specifically reacted with HHV-7-seropositive sera. This reactivity was significantly inhibited by the preincubation of sera with the peptide itself, lysates of gB-expressing cells, or lysates of HHV-7-infected cells. The reactivity was not significantly modified when sera were preincubated with lysates of either human cytomegalovirus (HCMV)- or HHV-6-infected cells. In cross-sectional studies including both children and adults, 49 out of 61 serum samples (80%) were found to be positive by HHV-7 ELISA, independent of their reactivity to HCMV. A longitudinal serological study of 17 children during the first 4 years of life showed that the level of ELISA-detected antibodies significantly decreased within a few weeks after birth and then increased in the following months, likely reflecting, respectively, the loss of maternal antibodies and the occurrence of seroconversion. These results demonstrate that gB peptide ELISA might be a useful tool for the serological study of HHV-7 infection.

Present address: LFB, Les Ulis, 91958 Courtaboeuf Cedex, France.

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